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Biotinylated Mouse PCSK9 Protein, Avitag™,His Tag

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PC9-M82E1-25ug
$360.00
In Stock - Delivery in 1-2 business days
PC9-M82E1-200ug
$1,565.00
In Stock - Delivery in 1-2 business days
PC9-M82E1
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Synonyms

PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9

Source

MABSol® Biotinylated Mouse PCSK9, Avitag,His Tag (PC9-M82E1) is expressed from human HEK293 cells. It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1).

Predicted N-terminus: Gln 35

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The protein has a calculated MW of 13.9 kDa and 61.1 kDa. The protein migrates as 16 kDa and 66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

バックグランド

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as RC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).