Biotinylated Human / Cynomolgus / Rhesus macaque ROR1 Protein, Fc,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
RO1-H82F4-25ug
$360.00
RO1-H82F4-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    ROR1, NTRKR1

  • Source

    Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Fc,Avitag (RO1-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1). In the region Gln 30 - Glu 403, the amino acid sequences of Human, Cynomolgus and Rhesus macaque ROR1 are identical.

    Predicted N-terminus: Gln 30

    Request for sequence
  • Molecular Characterization

    ROR1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 70.1 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ROR1 SDS-PAGE

    Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    ROR1 SEC-MALS

    The purity of Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Fc,Avitag (Cat. No. RO1-H82F4) is more than 95% and the molecular weight of this protein is around 160-195 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ROR1 ELISA

    Immobilized Anti-ROR1 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Fc,Avitag (Cat. No. RO1-H82F4) with a linear range of 0.2-6 ng/mL (QC tested).

    Protocol
  • Bioactivity-FACS

     ROR1 FACS

    2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Fc,Avitag (Cat. No. RO1-H82F4) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

    Protocol

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Background

Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.

Recent Advances

 
Drug Development Progress
  • English Name:

    Inactive tyrosine-protein kinase transmembrane receptor ROR1

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    22 Details

  • Highest Development Stage:

    Phase 3 Clinical

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