Biotinylated Human Syndecan-1 Protein, His,Avitag™

製品番号/仕様
価格
数量
SY1-H82E2-25ug
$360.00
SY1-H82E2-200ug
$1565.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    SDC1, Syndecan-1, CD138, SYND1, SDC

  • Source

    Biotinylated Human Syndecan-1, His,Avitag (SY1-H82E2) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).

    Predicted N-terminus: Gln 23

    Request for sequence
  • Molecular Characterization

    Syndecan-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 27.5 kDa. The protein migrates as 44-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Syndecan-1 SDS-PAGE

    Biotinylated Human Syndecan-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     Syndecan-1 ELISA

    Immobilized Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate, can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.4-6 ng/mL (QC tested).

    Protocol
  •  Syndecan-1 ELISA

    Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) with a linear range of 2-63 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-FACS

     Syndecan-1 FACS

    2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

    Protocol

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バックグランド

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Syndecan-1

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    2 詳細

  • 最新の開発ステージ:

    Phase 1 Clinical

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