Biotinylated Human Latent TGF-beta 3 / Latent TGFB3 Protein, His,Avitag™

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製品番号/仕様
価格
数量
TG3-H82E6-20ug
$360.00
TG3-H82E6-200ug
$1815.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    TGFB3, ARVD, TGF-beta3

  • Source

    Biotinylated Human Latent TGFB3, His,Avitag (TG3-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Ser 412 (Accession # P10600-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    TGF-beta 3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 48.4 kDa. The protein migrates as 15 kDa, 40-45 kDa and 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    TGF-beta 3 SDS-PAGE

    Biotinylated Human Latent TGFB3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     TGF-beta 3 ELISA

    Immobilized Human TGFBR2, Fc Tag (Cat. No. TG2-H5252) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGFB3, His,Avitag (Cat. No. TG3-H82E6) with a linear range of 0.3-20 ng/mL (QC tested).

    Protocol

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バックグランド

Transforming growth factor beta 3 ( TGFB3) is also known as TGF-β3, is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. TGF-beta 3 is similar with TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Transforming growth factor beta 3

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    3 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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