user
menu
letter
Sign InRegister
langEnglisharrow

Solutions for RSV vaccine and drug development

Human Respiratory Syncytial Virus, (HRSV) is a negative-stranded RNA virus of the Pneumoviridae family (genus Orthopneumovirus) that can inflict potentially deadly respiratory tract infections in infants, the elderly, and immunocompromised individuals leading to approximately 50,000 deaths of children under 5 per year.

The HRSV genome is about 15.2kb in length and encodes for 11 proteins. RSV surface fusion protein (F) and attachment (G) glycoproteins play important roles in mediating infection and are both major targets of the humoral immune response. RSV F is well conserved while G is highly variable, with the two antigenic subgroups of RSV (A and B) being defined primarily by differences in the G protein. Vaccine and therapeutics development efforts have largely focused on RSV F.

Solutions for RSV vaccine and drug development
Solutions for RSV vaccine and drug development

（10.1038/s41579-019-0149-x）

RSV F is a class I fusion protein that rearranges from a metastable prefusion conformation to a highly stable postfusion structure. Based on the structural research on RSV F protein, there are at least 7 different antigenic epitopes that have been identified, termed Site Ø, I, II, III, IV, V, and a quaternary dependent (QD) site. Of these sites, only I, II, and IV are exposed on the post-F form. The post-F form has been found to elicit relatively weaker neutralizing antibodies that fail to stop infection. The pre-fusion form has been proven to induce more efficient neutralizing antibodies, while the post-fusion form can be used as a control for screening for pre-specific antibody.
The epitopes of RSV-F protein and antibody binding
The epitopes of RSV-F protein and antibody binding
ACROBiosystems provides comprehensive solutions to support RSV vaccine and drug development efforts!

RSV vaccines and drugs R&D solutions

Kits
Antigens
Antibodies
Cat. No.MoleculeProduct DescriptionOrder Now
Order
Order
Order
Order
Order
Order
Order
Order

Main Features

Specially designed natural conformation: Specially designed trimeric Pre-F protein, Post-F protein, and G protein with natural trimeric conformation.

Rich product types: Covers RSV Pre-F, Post-F and G proteins with correct conformation, RSV-F0 broad-spectrum antibodies and Pre-F0 specific antibodies, Pre-F0 antigen and antibody detection kits;

High purity: Purity >90% verified by SDS-PAGE and SEC-MALS.

High activity: Strong binding ability of Pre-F and Post-F proteins to their specific antibodies verified by ELISA.

High immunogenicity: Natural conformation RSV-F and G proteins can induce higher antibody titers.

Assay Data

Natural trimeric Pre-F protein validation data
SEC-MALS verified Pre-F trimeric protein purity >90%
Natural trimeric Pre-F protein validation data

The purity of HRSV (A) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) is more than 90% and the molecular weight of this protein is around 175-200 kDa verified by SEC-MALS.

ELISA verified Pre-F trimeric protein binding with Pre-F specific antibody (D25) linear range 0.2-1 ng/mL
Natural trimeric Pre-F protein validation data

Immobilized HRSV (A) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) with a linear range of 0.2-1 ng/mL (QC tested).

Pre-F specific antibody product validation data
ELISA verified Pre-F specific antibody 12C6 can specifically recognize Pre-F trimer, not binding to Post-F trimer
Pre-F specific antibody product validation data

Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-Pre-F0 specific Antibody, Human IgG1 (12C6) (Cat. No. RS0-S286) with a linear range of 0.2-2 ng/mL.HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is not recognized by Monoclonal Anti-RSV-Pre-F0 specific Antibody, Human IgG1 (12C6) (Cat. No. RS0-S286), confirming specificity for the pre-fusion conformation.

ELISA Detection Kits
ELISA Detection Kits

Detection of Pre-Fusion glycoprotein F0 (RSV) by sandwich-ELISA Assay.Immobilized Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody can bind Pre-Fusion glycoprotein F0 (RSV). Detection was performed using HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody with sensitivity of 3.1 ng/mL (QC tested).

Resources

Resources
Solutions for RSV vaccine and drug development
Analytical Methods to Identify Structural Conformation of RSV Pre- and Post-fusion Proteins for Vaccine Development

FAQ

Q

What RSV product types are available for vaccine and drug development?

The range includes pre-fusion and post-fusion F proteins, RSV A and B G proteins, monoclonal antibodies, F-antigen detection kits and anti-RSV-F antibody-titer kits.
Q

Why does the choice between pre-fusion and post-fusion RSV F matter?

Pre-F and post-F present different epitope landscapes. Pre-F retains neutralization-sensitive sites that are not all exposed after fusion, while post-F can serve as a control in pre-F-specific antibody screening.
Q

Are products available for both RSV A and RSV B?

Yes. RSV A and RSV B options are listed for pre-F, post-F and G proteins, and selected RSV B-specific kits and antibodies are also included.
Q

Can I select RSV G protein by antigenic subgroup?

Yes. His-tagged G proteins are listed for both RSV A and RSV B. Choose the subgroup that matches the viral strain or antibody specificity being studied.
Q

Which ELISA kits distinguish specific RSV F conformations or epitopes?

The listed kits include pre-F-specific, post-F-specific and RSV B pre-F assays, with some pre-F kits directed to sites such as site Ø, site V or both. Select by conformation and epitope requirement.
Q

Are antibody-titer kits available for both human and mouse samples?

Yes. Separate human and mouse anti-RSV-F0 IgG titer ELISA kits using pre-fusion F0 are listed. Use the kit validated for the study species.
Q

Can I obtain antibodies that distinguish pre-F from post-F?

Yes. The antibody range includes pre-F-specific and post-F-specific reagents as well as broader F0 antibodies. A selected pre-F antibody is shown recognizing pre-F without binding post-F under the tested ELISA conditions.
Q

What structural and activity data are available for RSV proteins?

Selected pre-F and post-F proteins have SDS-PAGE, SEC-MALS and ELISA binding data. For example, the listed RSV A pre-F product RSF-V52H7 is described as a trimer with greater than 90% purity; verify each catalog number separately.
  • Background
  • RSV vaccines and drugs R&D solutions
  • Main Features
  • Assay Data
  • Resources
  • FAQ