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Cell Phenotyping Assay

ACROBiosystems offers a diverse range of flow cytometry analysis solutions, built on a rigorously validated platform to precisely identify cell composition and status. Our product includes a wide selection of flow cytometry antibodies labeled with various fluorochromes, as well as ready-to-use premixed kits, designed to meet your needs across different flow cytometers. These high-quality, high-efficiency tools empower your immunophenotyping analysis in applications such as preclinical drug development and immune monitoring in clinical trials.

For more fluorescent dye labeling or color customization options, please click Inquire.

Flexible selection of fluorescent antibodies
Rapid Detection in Just 15 Minutes
Testing Workflow Diagram
Testing Workflow Diagram

Key Advantages

Flexible Selection:Offers both antibodies and kits, allowing flexible selection of fluorescent antibodies to analyses different cells.

Lightning-Fast Result: Achieve reliable staining in just 15 minutes with our one-step mixed antibody cocktail

Effortless Operation: Simply mix, incubation, and detect with a flow cytometer.

Universal Compatibility: Suitable for flow cytometers with blue and red lasers and six-color detection channels.

High Throughput: Capable of analyzing up to 50 samples simultaneously.

High Specificity: Validated with Isotype Control, FMO Control, and Mock Cell Controls.

Optimal Sensitively: Specially selected antibody and dye combinations ensure precise detection.

Reliable data validation:Verified by real PBMC samples

Cost-Effective: Proprietary antibody and fluorochrome coupling technology.

Product List

Fluorescent Antibody
Premixed Panel
Antibody Labeling Kits
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Product Performance

Antibody Performance Data
APC-Labeled Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3)

Flow cytometric analysis of Jurkat cells staining with APC-Labeled Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (Cat. No. CDE-AHFP1) at 1:50 dilution (2μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) , compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).

PE-Labeled Monoclonal Anti-Human HLA-DR Antibody, Mouse IgG2a(L243)

Flow cytometric analysis of Raji staining with PE-Labeled Monoclonal Anti-Human HLA-DR Antibody, Mouse IgG2a(L243) (Cat. No. FABm020-01) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

Panel Performance Data
Proven reliability with real PBMC samples
Start by setting the CD45/SSC scatter plot to identify the CD45+ lymphocyte population (A). Within the CD45+ gate, use the CD3/SSC scatter plot to identify the CD3+ T cell population (B). Next, use the CD3/CD4 scatter plot to identify the CD3+CD4+ helper T cells (Th gate) (C), and the CD3/CD8 scatter plot to identify the CD3+CD8+ cytotoxic T cells (Tc gate) (D). For B cells, use the CD3/CD19 scatter plot to identify the CD3-CD19+ population (B gate) (E). Finally, set the CD3/CD16+CD56 scatter plot to assess the CD3-CD56+CD16+ NK cell population (F).
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FAQ

Q

Why is precise cell phenotyping increasingly critical in modern drug development?

Precise cell phenotyping allows researchers to identify specific cell compositions and statuses with high accuracy. In applications such as preclinical drug development and immune monitoring during clinical trials, understanding the exact population dynamics of immune cells is essential for evaluating therapeutic efficacy and safety.
Q

Should I choose individual fluorescent antibodies or a premixed panel for my study?

Individual fluorescent antibodies are ideal for researchers building customized multicolor panels or optimizing specific assays. Ready-to-use panels provide pre-optimized antibody combinations that simplify workflow, reduce assay variability, and improve reproducibility. For standardized applications such as TBNK immunophenotyping, a premixed panel is typically the more effective choice.
Q

Which flow cytometers are compatible with ACRO's cell phenotyping reagents?

ACRO's fluorescent antibodies and premixed panels are validated for flow cytometers equipped with blue and red lasers and six-color detection channels. For laboratories using alternative instrument configurations, fluorochrome customization is also available to support different panel designs.
Q

How can I reduce non-specific staining in flow cytometry assays?

ACRO validates its reagents using Isotype Controls, Fluorescence Minus One (FMO) Controls, and Mock Cell Controls to verify staining specificity and gating accuracy. These validation strategies help minimize non-specific signals and improve confidence when identifying target cell populations.
Q

How does ACRO ensure lot-to-lot consistency of its fluorescent antibodies?

ACRO utilizes proprietary antibody conjugation technology to ensure consistent fluorochrome labeling while maintaining antibody specificity and binding performance. Each production lot is validated using real PBMC samples to confirm consistent staining quality, delivering high lot-to-lot reproducibility and reliable performance across experiments.
Q

Can ACRO's cell phenotyping assays support high-throughput workflows?

Yes. ACRO's cell phenotyping assays feature a streamlined one-step staining protocol that can be completed in approximately 15 minutes. The workflow supports simultaneous processing of up to 50 samples, enabling efficient, standardized immune cell analysis while maintaining high data quality and assay reproducibility.
Q

What are the advantages of the Human TBNK 6-color Cocktail Flow Panel?

The Human TBNK 6-color Cocktail Flow Panel contains pre-optimized fluorescent antibodies for simultaneous identification of T cells, B cells, NK cells, CD4⁺ T cells, and CD8⁺ T cells in a single assay. The ready-to-use format eliminates manual antibody preparation, shortens assay setup time, and improves inter-assay consistency, making it well suited for standardized immune cell profiling.
Q

When should I opt for an "Antibody Labeling Kit" instead of a pre-labeled antibody?

If you are working with a unique or proprietary antibody that is not available in a pre-labeled format, ACRO’s Antibody Labeling Kits (FITC, APC, or R-PE) are the ideal choice. These kits allow you to label your own antibodies in-house while benefiting from our high-performance fluorochromes and reliable labeling protocols.
  • Background
  • Key Advantages
  • Product List
  • Product Performance
  • FAQ