English

Extracellular Matrix (ECM) Proteins

Extracellular matrix (ECM) is primarily composed of collagen, glycoproteins (such as laminin, vitronectin, and fibronectin), and proteoglycans. It is crucial for stem cell adhesion and tissue stability by activating signaling pathways through integrins. For effective use in regenerative medicine and cell therapy, chemically defined, non-xenogeneic, reproducible, and scalable culture conditions are required. This is typically achieved by adding specific ECM components into the culture system, providing a stable environment essential for stem cell survival.

Under a well-established cell culture platform and stringent quality management system, we proudly launch our PG(Pre-GMP)-grade and GMP-grade Laminin, Fibronectin, Vitronectin, and other ECM protein products. These provide an optimal extracellular matrix microenvironment for stem cells such as hPSCs and MSCs, supporting the entire workflow from early-stage R&D to clinical translation.

Moreover, our Laminin products strictly adhere to internationally leading viral safety control standards, implementing a comprehensive safety assurance system through five core strategies:

Components and functions of stem cell niches.
Nat Rev Mol Cell Biol 9, 11–21 (2008).

Rigorous Cell Bank Testing

Comprehensive testing (28 items) of the adapted and banked production host cell line, performed by internationally renowned third-party testing agencies in compliance with US and Chinese Pharmacopoeia standards and ICH Q5.

Animal-Origin-Free (AOF) Production Process

Upstream culture uses chemically defined medium (CDM), with all raw materials being serum-free (SF) and animal-origin-free (AOF), thoroughly eliminating the risk of animal-derived viral contamination.

Dual Virus Reduction Measures

Incorporation of viral clearance/inactivation steps such as low-pH treatment and nanofiltration in the downstream purification process.

Comprehensive Viral Clearance Validation

Validation of key viral removal steps through dedicated viral clearance studies.

Stringent Final Product Quality Control and Release

Products undergo testing for adventitious agents, multiple process-related impurities, and a total of 13–16 QC release tests.

Search our ECM Proteins

We provide a comprehensive selection of validated, high-activity extracellular matrix proteins, including Laminin, Vitronectin, and Fibronectin. These proteins are specifically designed to support a wide range of stem cell culture requirements and have been tested to ensure they meet the highest standards, offering exceptional performance and reliability for diverse applications in stem cell research and therapy.

Matrix Proteins	Catalog No.	Product	Application
Laminin 521	GMP-LA5H24GA02	GMP Human Laminin 521 Protein (Liquid)	Human PSC expansion in the clinical stage
	GMP-LA5H24	GMP Human Laminin 521 Protein	Human PSC expansion in the clinical stage
	LA5-H5217	Human Laminin 521 Protein, premium grade (Flagship Pro)	Human PSC expansion in the pre-clinical stage
	LA5-H5213L	Human Laminin 521 Protein, premium grade (Liquid)	Human PSC expansion in the pre-clinical stage
	LA5-H5261	Human Laminin 521 Protein, premium grade	Human PSC expansion in the pre-clinical stage
	LA5-H5248	Human Laminin 521 Protein, premium grade (Flagship)	Human PSC expansion in the pre-clinical stage
Laminin 511	GMP-LA1H36	GMP Human Laminin 511 Protein (Mix & Scale)	Human PSC expansion in the clinical stage
	GMP-LA1H25	GMP Human Laminin 511 Protein	Human PSC expansion in the clinical stage
	LA1-H5314	Human Laminin 511 Protein, premium grade (Mix & Scale)	Human PSC expansion in the pre-clinical stage
	LA8-H5283L	Human Laminin 511 Protein, premium grade	Human PSC expansion in the pre-clinical stage
Vitronectin	GMP-VINH19	GMP Human Truncated Vitronectin / VTN-N Protein	Human PSC expansion in the clinical stage
Vitronectin	VIN-H5117	Human Truncated Vitronectin / VTN-N Protein, premium grade	Human PSC expansion in the pre-clinical stage
Fibronectin	GMP-FINH18	GMP Recombinant Human Fibronectin fragment	Mesenchymal stem cell attachment and proliferation
Fibronectin	FIN-H5116	Recombinant Fibronectin fragment, premium grade	Mesenchymal stem cell attachment and proliferation
Laminin 111	LA1-H5269	Human Laminin 111 Protein, premium grade	Hepatic and neural differentiation
Laminin 121	LA1-H5261	Human Laminin 121 Protein, premium grade	Especially hepatic and neural cells among many other cell types
Laminin 211	LA8-H5263	Human Laminin 211 Protein, premium grade	Skeletal muscle, neural and cardiac cells differentiation
Laminin 221	LA2-H5269	Human Laminin 221 Protein, premium grade	Cardiac cells differentiation
Laminin 411	LAT-H5263	Human Laminin 411 Protein, premium grade	Endothelial and pancreatic cells differentiation

Can't find the
Product you need?

Request Custom Services

Making the Transition to GMP

Delivering high-quality GMP products at an affordable cost lowers barriers to accessing innovative medicines, including cell therapies. Transitioning from our premium (Pre-GMP) grade products to GMP requires minimal comparability testing. Our premium(Pre-GMP)-grade products have validated equivalent bioactivity and consistency and are manufactured using the same methods and processes. Learn more about GMP vs Premium(Pre-GMP) Grade

Empowering Academia — Research Grade Human Laminin 521 Protein

We are now offering a special promotion of Research Grade (RG) Human Laminin 521, exclusively for our academic customers. This initiative is a token of our gratitude for the extensive support from research institutions and aims to further advance foundational stem cell research. RG Laminin 521 is now available at a more affordable price, and we welcome your inquiries and orders.

Performance

Featured Product——GMP grade Laminin 521

Performance - facilitate rapid expansion of hPSC and support efficient differentiation into a diverse array of specialized cell types.
Better Adhesion - maintain good adhesion characteristics at a concentration as low as 2μg/ml.
Stemness Maintenance - no spontaneous differentiation is observed after several passages of hPSC culture.
Lot-to-Lot Consistency - produced from a stable cell line, robust purification process, stringent QC.
Ready to Scale-up Supply - cGMP-compliant facility.

Laminin 521 (GMP-LA5H24) effectively maintains the expansion of human iPSCs.

Laminin 521 (GMP-LA5H24) could maintain the stemness of iPSC after several passages.

Normal karyotype (46, XX) was found in hiPSCs with Laminin 521(GMP-LA5H24) coating after 10 passages.

Featured Product——GMP grade Fibronectin

Expressed using a recombinant E. coli system, ensuring enhanced safety profile.
Exhibits high batch-to-batch consistency, suitable for large-scale production.
Demonstrates high biological activity, validated through stem cell culture.

GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) could promote mesenchymal stem cell attachment and proliferation in several passages, comparable with Company T. The bioactivity of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116).

GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) could maintain the stemness of MSC in several passages, with high expression of MSC markers CD73, CD90 and CD105 and negative expression of HLA-DR, CD34 and CD45. The stemness ability of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is comparable with Company T and similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116).

CD34+ CD45+ hematopoietic cells were seeded on GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) coated plates and differentiated for 21 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD5 and CD7. GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) can induce the formation of CD7+ and CD5+ CD7+ T-cell progenitors. The bioactivity of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116), as well as the Company T. Negative control: w/o Human Fibronectin.

Interested in testing
a GMP grade product?

Request a GMP Sample

Frequently Asked Questions

1 How to choose the different grades for Laminin 521?

For academic customers, we recommend research grade Laminin 521, which is suited for stem cell research and highly affordable compared to other commercial extracellular cell matrixes. GMP grade laminin 521 is designed for large scale manufacturing process of iPSC in the clinical stage, which is produced under strict GMP systems and comply with regulations from multiple countries.

2 What is the advantage of recombinant Laminin 521 compared to other commercial basement membrane extracts?

Recombinant Laminin 521 is the defined cell matrix with good lot-to-lot consistency. It supports faster stem cell growth in monogenous monolayer compared to basement membrane extracts. It is widely used for in vitro feeder-free culture of multiple human pluripotent stem cells and maintain the high quality.

3 How to transfer my stem cells from other commercial extracellular matrix proteins, such as Matrigel, Laminin to our Laminin 521?

No more special adaption process is needed when you transfer your stem cells from other commercial extracellular matrix proteins, such as Matrigel, Vitronectin and other Laminin proteins to our Laminin 521 protein. We recommend adapting your stem cells by at least 2 passages and adding Rock inhibitor (such as Y-27632). If it is hard to adapt, the higher working concentration of Laminin 521 protein (such as 10 μg/ml) is recommended. After several passages, if your stem cells are well adapted, you can reduce the working concentration and cell density.

4 How to passage my stem cell within Laminin 521 coating?

We have tried different dissociation enzymes, such as EDTA, Accutase, TrypLE Select to passage iPSC. Generally, the incubation time is about 5-6 minutes and will be longer depending on special cell lines. Meanwhile, stem cells coated with Laminin 521 often attach tighter compared to other matrix proteins. The lower working concentration of Laminin protein should be used to reduce the detach time.

5 How to choose different kinds of laminin protein for stem cell expansion and differentiation?

Laminin proteins are crucial for stem cell expansion and defined cell differentiation based on the expression in different tissues in human body. For human pluripotent stem cell culture, Laminin 521 and Laminin 511 are recommended for the expansion of high-quality stem cells. Laminin 111 and Laminin 121 are used in hepatic and neural cell differentiation. Laminin 211 could support skeletal muscle, neural and cardiac cells differentiation. Laminin 411 is used in endothelial and pancreatic cells differentiation.

Resources

Literature

Flyer—GMP Grade Laminin 521

Flyer—Research Grade Human Laminin 521

Brochure—Induced Pluripotent Stem Cells Culture and Differentiation Solutions

Brochure—Making the Transition to GMP

FAQ

What is the extracellular matrix (ECM), and why is it vital for stem cell survival and function?

The extracellular matrix (ECM) is a complex network primarily composed of collagens, glycoproteins (such as laminin, vitronectin, and fibronectin), and proteoglycans. In stem cell biology, the ECM does more than provide a physical scaffold; it acts as a critical signaling hub by activating specific pathways through integrins. Providing a stable and chemically defined ECM environment is essential for maintaining cell adhesion, regulating differentiation, and ensuring the survival of stem cells in in vitro culture systems.

How do ACRO’s recombinant ECM proteins improve experimental reproducibility compared to traditional basement membrane extracts (BME)?

Traditional animal-derived BMEs are often ill-defined and suffer from significant batch-to-batch variability, which can compromise the reliability of stem cell research. ACROBiosystems offers recombinant, chemically defined, and non-xenogeneic ECM proteins that provide unmatched lot-to-lot consistency. These products support faster, more homogeneous monolayer growth and ensure that researchers can achieve reproducible results across different stages of their workflow, from early discovery to large-scale clinical manufacturing.

Can recombinant Fibronectin support the large-scale expansion of Mesenchymal Stem Cells (MSCs) without losing their stemness?

Yes. ACRO’s GMP Recombinant Human Fibronectin fragment is specifically expressed in a recombinant E. coli system to ensure high purity and a superior safety profile. It has been validated to promote the attachment and rapid proliferation of MSCs while maintaining the high expression of essential stemness markers, such as CD73, CD90, and CD105. This makes it a stable and high-performance benchmark for researchers transitioning to large-scale MSC production for regenerative medicine.

What are the operational differences between the Pre-coating and Suspension methods for iPSC culture?

The choice depends on the scale and automation needs of your project: Pre-coating Method (Laminin 521): This approach involves coating plates before cell seeding, which promotes high cell motility and excellent clonal survival, resulting in a tightly arranged monolayer. Suspension Method (Laminin 511/Mix & Scale): This innovative approach eliminates the need for plate coating, significantly reducing material usage and curing times. It is automation-ready and highly compatible with high-throughput systems, making it the ideal choice for researchers looking to optimize and simplify their cultivation workflows.

How does ACROBiosystems ensure the viral safety of ECM proteins for clinical-grade manufacturing?

Safety is paramount in clinical translation. ACRO implements a comprehensive safety assurance system based on five core strategies: Rigorous Cell Bank Testing: Production host cell lines undergo a 28-item testing panel in compliance with US/Chinese Pharmacopoeia and ICH Q5 standards. Animal-Origin-Free (AOF) Production: Upstream cultures use chemically defined, serum-free, and AOF media to eliminate the risk of animal-derived contamination. Dual Virus Reduction: The downstream process includes low-pH treatment and nanofiltration for viral inactivation. Clearance Validation: Key steps are verified through dedicated viral clearance studies. Stringent QC Release: Final products undergo 13–16 separate QC release tests for adventitious agents and process-related impurities.

Why is a chemically defined recombinant matrix preferred over traditional basement membrane extracts (BME) for high-quality iPSC expansion?

Traditional BMEs are often animal-derived and ill-defined, leading to significant batch-to-batch variability and potential contamination risks. Recombinant matrices, such as Laminin 521, provide a well-defined environment with unmatched lot-to-lot consistency. This supports faster, more homogeneous monolayer growth and effectively maintains stemness and a normal karyotype across several passages, which is critical for clinical-grade cell therapy development.

How does ACROBiosystems simplify the transition from early-stage R&D to large-scale clinical manufacturing?

To lower the barriers to clinical translation, ACRO provides matched grades of ECM proteins: Premium (Pre-GMP) and GMP. Because both grades are manufactured using the same stable cell lines and processes, they exhibit equivalent bioactivity and consistency. This allow researchers to validate their research in the pre-clinical stage with minimal comparability testing required when scaling up to GMP-compliant manufacturing.

How do ACROBiosystems' recombinant laminins support the maintenance of iPSC pluripotency? What data demonstrate their performance?

ACROBiosystems' Laminin 521 has been extensively validated through comprehensive cell-based studies to demonstrate its ability to support long-term iPSC culture: Stable pluripotency marker expression: After continuous passaging, iPSCs maintain high expression of key pluripotency markers, including OCT4, SOX2, and Nanog, with no evidence of spontaneous differentiation. Reliable genomic stability: Following more than 10 consecutive passages, flow cytometry and karyotype analyses confirm normal chromosomal integrity with no detectable abnormalities. Robust trilineage differentiation potential: Cultured iPSCs retain their ability to differentiate efficiently into functional cell types derived from the ectoderm, mesoderm, and endoderm. In addition, ACROBiosystems' recombinant laminins are manufactured using an animal origin-free (AOF) process with multiple viral clearance steps, ensuring excellent batch-to-batch consistency and reliable performance. These features make them well suited for both basic stem cell research and iPSC-based cell therapy development, while supporting the quality requirements for clinical and regulatory applications.

Introduction
Search our ECM Proteins
Performance
Frequently Asked Questions
Resources
FAQ