Inhibitor Screening Kits

Inhibition of PD-1: PD-L1 binding by anti-PD-1 neutralizing antibody measured using the PD-1 [biotinylated]: PD-L1 inhibitor screening ELISA assay pair (Cat. No. EP-101).

Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

Inhibition of TL1A [Biotinylated] : DR3 binding by anti-TL1A neutralizing antibody measured using TL1A [Biotinylated] : DR3 inhibitor screening kit (Cat. No. EP-168)

Serial dilutions of Human Anti-TL1A Neutralizing antibody (Catalog # EP168-C04) (1:1 serial dilution, from 5 μg/mL to0.0390625 μg/mL) was added into Human DR3: Biotinylated Human TL1A binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The example data is for reference only.

Inhibition of IL-4: IL-4 R alpha binding by Human IL-4 measured using IL-4 [biotinylated]: IL-4 R alpha inhibitor screening kit (Cat. No. EP-132).

Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

Inhibition of Integrin α 4 β 7: MAdCAM-1binding by anti-Integrin α 4 β 7 neutralizing antibody measured using Integrin α 4 β 7: MAdCAM-1 [Biotinylated] inhibitor screening kit (Cat. No. EP-155)

Serial dilutions of Anti-Integrin α 4 β 7 Neutralizing antibody (Catalog # EP155-C04) (1:1 serial dilution, from 5 μg/mL to 0.0098μg/mL) was added into Integrin α 4 β 7: Biotinylated MAdCAM-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).