Enzymes
Target enzyme
Enzymes are catalysts in organisms that regulate chemical reactions without undergoing changes themselves. They are important biological targets for drug development, offering unique opportunities for drug design. Enzyme inhibitors play a crucial role in developing oral therapeutic agents used in clinical practice.
ACROBiosystems offers a range of target enzyme products for drug development. These include targets in adenosinergic pathways, matrix metalloproteinases, lysosomal proteases, and kinases. These products support the development of drugs and therapies targeting these enzymes.
Product Features
Proteins with multiple species, including human, mouse, cynomolgus, rabbit, and canine, are suitable for cross-species experiments.
Verified high purity: more than 95% as verified by SDS-PAGE; more than 90% as verified by SEC-MALS.
Enhanced enzymatic activity for improved substrate catalysis and drug screening.
Reliable and reproducible results with consistent batch-to-batch performance.
Verified high purity: more than 95% as verified by SDS-PAGE; more than 90% as verified by SEC-MALS.
Enhanced enzymatic activity for improved substrate catalysis and drug screening.
Reliable and reproducible results with consistent batch-to-batch performance.
Featured products
The critical targets for adenosinergic pathways
KRAS proteases
Cathepsin B proteases
Applications
High purity verified by SEC-MALS
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- The purity of Human CD39, His Tag (Cat. No. CD9-H52H4) is more than 90% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.
Enzyme activity data
| Product | Human Cathepsin B proteases / CTSB Protein, His Tag (active enzyme)(Cat.No. CTB-H5222) |
| Substrate | Fluorogenic peptide substrate Z-LR-AMC |
|
Enzyme Activity (pmol/min/μg) |
> 2500 |
| Product | Human KRAS (2-185,G12C) Protein, His Tag (active enzyme)(Cat.No. KRS-H51H3) |
| Substrate | GTPase-Glo test kras activity |
| Enzyme Activity | GTP≤85% |
Target enzyme Product List
- The critical enzyme for adenosinergic pathways
- Protease
- Kinase
- Alkaline phosphatase-like enzyme
- Others
The critical enzyme for adenosinergic pathways
> (Click molecular to learn more details)
Protease
> Matrix metalloproteinases
> Cathepsins
> Coagulation factors
Coagulation Factor III Coagulation factor VII Coagulation factor IX Coagulation factor X Coagulation factor XI
> ADAM proteases
> Others
Kinase
> Tyrosine kinase
> Receptor tyrosine kinase
AXL DDR2 EGFR EGFRvIII EphA2 EphA4 EphA5 EphA7 EphA10 EphB4 FGF R1 FGF R2 (IIIb) FGF R2 (IIIc) FGF R3 (IIIb) FGF R3 (IIIc) FGF R4 FGF R5 Flt-3 Her2 PDGF R alpha PDGF R beta MERTK TrkA TrkB TYRO3
> Serine/threonine-protein kinase
Others
| Transglutaminases | Protein Arginine Deiminase | PADI2 PADI3 PADI6 |
| Ubiquitin Related Proteins | CBLB Elongin B & Elongin C & VHL UBE1 UCH-L1 UCH-L3 RNF43 | |
| Oxidoreductases | IDH1 TDO2 Xanthine dehydrogenase | |
| Hydrolases | Angiogenin beta-Galactosidase-1 beta-Glucuronidase/GUSB Glucosylceramidase Matriptase PH20 Plasma Kallikrein/KLKB1 Prostatic Acid Phosphatase | |
| Phosphatase | PTPRD | |
| Carbonic Anhydrase | Carbonic Anhydrase II Carbonic Anhydrase IX Carbonic Anhydrase XII | |
| Others | Plexin B1 Plexin B2 GAS6 | |
- The critical enzyme for adenosinergic pathways
- Protease
- Kinase
- Alkaline phosphatase-like enzyme
- Others
The critical enzyme for adenosinergic pathways
> (Click molecular to learn more details)
Tool enzyme
Tool enzymes, including nucleic acid enzymes, have diverse functions like restriction, polymerization, ligation, modification, and cleavage. They efficiently recognize specific sequences and enable the manipulation and analysis of nucleic acids. These enzymes are widely used to detect and study biologically active molecules.
ACROBiosystems offers CAS nucleases, including universal nucleases, CAS9, and CAS12a, for cell and gene engineering, as well as regulatory safety monitoring of biological products.
ACROBiosystems also provides tool enzymes for antibody analysis, aiding in antibody fragmentation, glycosylation analysis, and exploring the relationship between antibody structure and function. These tools contribute to the development and quality control of biopharmaceuticals.
Product Features
High Purity, as determined by SDS-PAGE (>90%) and SEC-MALS
Verified high enzymatic activity
Animal component-free
Verified high enzymatic activity
Animal component-free
Featured products
GENIUS™ Nuclease
Cas Enzyme
Enzymes for Antibody Characterization
> Nuclease
| Type | Molecular | Cat. No. | Description |
|---|---|---|---|
| GENIUS™ Nuclease | Nuclease HOT | GMP-NUES13 | GMP Salt Active GENIUS™Nucleas |
| Nuclease HOT | GMP-NUES19 | GMP GENIUS™Nuclease DMF Filed | |
| Nuclease HOT | BEE-N3116 | GENIUS™Nuclease DMF Filed | |
| Nuclease HOT | NUE-S5117 | Salt Active GENIUS™Nuclease, premium grade | |
| Nuclease HOT | NUE-S5119 | GENIUS™ Nuclease, premium grade | |
| Cas Enzyme | Cas9 | GMP-CA9S18 | GMP GENPower™ NLS-Cas9 Nuclease |
| Cas9 | CA9-S5149 | GENPower™ NLS-Cas9 Nuclease (MALS verified) | |
| Cas12a | CAA-A5143 | AsCas12a Nuclease (active enzyme, MALS verified) | |
| Cas12a | CAA-L5140 | GENPower™ NLS-Cas12a Nuclease (MALS verified) | |
| Cas12a | CAA-L5149 | NLS-Cas12a Nuclease (MALS verified) |
> Protease
| Type | Molecular | Cat. No. | Description | Application |
|---|---|---|---|---|
| Protease | 3C(HRV) | 3CC-N3133 | HRV-3C Protease Cleavage Enzyme, GST Tag (active enzyme) | HRV-3C removes affinity tag from fusion protein. |
| Enzymes for Antibody Characterization | IdeS | IDS-S5143 | IdeS (20U/μl) | IdeS specifically cleaves IgGs below the hinge region into F(ab´)2 and Fc fragments. |
| SpeB | SPB-S5115 | SpeB (40U/μl) | SpeB digests IgG to generate Fab and Fc fragments. | |
| Endo H | ENH-S5116 | Endo H (500U/μl) | Endo H cleaves asparagine-linked (N-linked) hybrid or high mannose oligosaccharides but not complex oligosaccharides. | |
| EndoS | ENS-S5143 | Endo S (200U/μl) | EndoS preferentially cleaves complex oligosaccharides and does not cleave asparagine-linked (N-linked) hybrid or high mannose oligosaccharides. |
Applications
The high enzyme activity is verified by in-vitro experiments
- NLS-Cas12a Nuclease (MALS verified) (Cat. No. CAA-L5149)substrate cutting efficiency>90%,the cleavage activity is comparable to that of competing products
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- Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage, comparable to competing products
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High bioactivity
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- GENIUS™ Nuclease, premium grade（Cat. No. NUE-S5119）shows higher specific activity than other competitors.
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High batch consistency
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- GENIUS™ Nuclease, premium grade (Cat. No. NUE-S5119), exhibits consistent bioactivity across different batches, ensuring high inter-batch uniformity.
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mRNA enzyme
During mRNA synthesis, modifications are employed to enhance translation efficiency, stability, and prevent degradation. These modifications involve capping the 5' end and appending a poly(A) tail at the 3' end of mRNA. ACROBiosystems offers enzyme products that facilitate mRNA synthesis and modification. These enzymes are of high purity, possess activity, and are free from contaminants. They effectively tackle challenges associated with capping efficiency, poly(A) tail sequences, mRNA stability, and cell delivery.
Product Features
Verified high purity
Verified high activity
Low endotoxin
Low dsRNA contamination
Verified high activity
Low endotoxin
Low dsRNA contamination
FAQ
How do recombinant target enzymes support drug discovery research?
Recombinant target enzymes are used in drug discovery studies to evaluate enzyme activity, target interactions, and inhibitor responses. ACROBiosystems provides recombinant enzymes across multiple target classes, including kinases, proteases, metalloproteases, adenosine pathway enzymes, and other disease-related enzymes. These products support applications such as biochemical assays, inhibitor screening, and target characterization.
What enzyme products support antibody characterization studies?
Tool enzymes support antibody characterization by enabling specific analytical approaches, including glycan analysis, IgG fragmentation, and protein processing. ACROBiosystems provides enzymes such as Endo H and Endo S for antibody N-glycan analysis, IdeS and SpeB for IgG fragmentation, and HRV-3C Protease for recombinant protein processing. The appropriate enzyme depends on the antibody attribute being analyzed and the intended characterization method.
How are recombinant nucleases and gene editing enzymes used in molecular research applications?
Recombinant nucleases support nucleic acid processing applications, while Cas-based enzymes support genome editing research. GENIUS™ Nuclease products are designed for nucleic acid removal applications, and NLS-tagged Cas9/Cas12a enzymes support genome editing studies in relevant research systems. Product selection depends on the required enzymatic function and experimental application.
What recombinant enzyme options are available for biopharmaceutical research applications?
ACROBiosystems provides recombinant enzymes for both target-based research and analytical applications. Target enzymes support studies such as inhibitor screening and functional activity assays, while tool enzymes support antibody analysis, nucleic acid processing, glycan characterization, and protein engineering workflows. Product-specific characteristics, including enzyme function and application compatibility, help researchers identify suitable reagents for their studies.
How do recombinant enzyme products support assay development and research evaluation?
Recombinant enzymes with characterized activity and purity information can support assay development and research evaluation across different applications. ACROBiosystems provides product-specific characterization data, including functional activity assessment and molecular characterization under reported assay conditions. These data help researchers evaluate compatibility with their intended experimental systems.
What role do enzymes play in enhancing the stability and translation efficiency of mRNA vaccines?
During mRNA synthesis, enzymes are required to add a 5' cap and a 3' poly(A) tail. ACRO’s specialized mRNA enzymes ensure high capping efficiency and tailing accuracy, which directly impacts the stability and translation performance of the mRNA once delivered to cells.
How can researchers integrate safety testing with enzymatic residue removal in the CMC process?
Removing residues is only half the task; you must also verify their absence. ACRO offers an integrated solution by providing high-performance enzymes for nucleic acid removal (like GENIUS™ Nuclease) alongside resDetect™ kits for detecting any remaining traces of the enzyme itself, ensuring a safe and cleared final formulation.
- Background
- Target enzyme
- Target enzyme Product List
- Tool enzyme
- mRNA enzyme
- FAQ