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Fc受容体タンパク質

Fc Receptor Proteins
製品情報

治療用抗体の有効性は、抗体のFabフラグメントと標的抗原への結合親和性だけでなく、FcフラグメントとFc受容体との結合によっても決定されます。FcフラグメントのFcRn(FCGRT&B2M)への結合親和性は抗体の半減期を予見する一方で、FcフラグメントとFCGRIIIA(CD16a)との結合により、抗体依存的細胞障害(antibody-dependent cell-mediated cytotoxicity; ADCC)が生じると考えられています。このように、抗体の研究においては、Fc受容体の解析は不可欠です。

弊社は抗体医薬品の開発をサポートするために、一般的な変異体を含む組み換え Fc 受容体タンパク質 コレクションを提供しております。

注目されているターゲット
                                                 
Fc受容体タンパク質製品の特長

HEK293細胞によって発現される: 翻訳後修飾の適切なタンパク質フォールディング

複数種の生物:ヒト、マウス、アカゲザル/アカゲザル、ラット、ブタ、ウサギ、ネコ、ウシなど様々な種間実験に完全に適しています。

高い純度:純度95%以上( SDS-PAGE による検証)純度90%以上(SEC-MALS による検証)

低エンドトキシン:< 1.0 EU / µg

安定性: ロット間の一貫性を確保するための厳格な品質管理が行われています。

AvitagTM で標識されたビオチン化Fc受容体タンパク質を提供可能:ラベル化効率が高く、標識部位が特異的で明確であるので、医薬品開発とプロセス最適化のための、ストレプトアビジンとの結合に基づくELISA / SPR / BLI検出法に適しています。

親和性はSPR & BLIによって確認されている:生物活性が保証できます。またプロトコルを無償で提供しております。

主要な機能

高純度

To meet the high purity requirement of pharmaceutical applications, our production team perform both SDS-PAGE and SEC-MALS analyses to the Fc receptors for QC purposes. It’s noteworthy that it’s essential to use monomeric Fc receptor, which represents its natural state. The formation of oligomers during production may result in artificially enhanced binding interaction due to avidity effect. To avoid such situation, we have established strict internal control standards of monomer purity by SEC-MALS testing. Only those batches meeting all purity requirements are released.

Human CD32a (R167), His Tag (Cat. No. CDA-H5221) MALS images

                                                   Fig. 1 The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 95% verified by SDS-PAGE. The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% verified by SEC-MALS. And the molecular weight of this protein is around 25-35 kDa.

生物活性はSPR&BLI法によって確認されている

The binding affinity between two molecules can be determined by many methods. However, the studies of Fc interaction are often done by SPR, because traditional ELISA is not appropriate for low affinity receptors such as CD16 and CD32. To assure our Fc receptors deliver expected performance, our QC team use Biacore platform to test our products against reference antibodies. Additionally, to meet more applications in the development of antibody drugs, our QC team also use ForteBio platform to test our products against reference antibodies. All SPR&BLI protocols are available for free.

FCA-H52H1-S1

Fig. 2 Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herc*ptin with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

BLIのプロトコルを申し込む
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Immobilized Human FcRn

Fig. 3 Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No.  FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herc*ptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).

SPRのプロトコルを申し込む
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Fig. 4 Loaded Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herc*ptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e).

BLIのプロトコルを申し込む
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Fig. 5 Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) on SA Biosensor via Biotin his antibody, can bind Herc*ptin with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e).

BLIのプロトコルを申し込む
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ロット間の一貫性は良好

We routinely apply rigorous quality control measures to ensure consistent performance of our product. As shown below, the batch variation among the tested samples is negligible.

1533000591779180.jpg

Fig. 6 Immobilized Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Ritux*mab with an affinity constant of 10 μM as determined in a SPR assay (Biacore T200).

SPRのプロトコルを申し込む
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We have compared the performance of different batches of CD32b (CDB-H5228) in the same assay, and the software analysis showed that the similarity score was very high, meaning high batch-to-batch consistency.

RIIB

Fig. 7 Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The consistency of different batches is more than 90%.

ビオチン化の、またはほかの種のFc受容体

The use of biotin labeling can make your assay development much easier. We offer a variety of ready-to-use biotinylated Fc receptors. These proteins are produced using our in-house developed labeling techniques, which confers high bioactivity and minimal batch-to-batch variation. In addition, we have developed a series of Fc receptors of other species, which are suitable for the screening of non-humanized antibodies or the species cross reaction.

human FcRn

Fig. 8 Biotinylated Human CD16a (V176), His Tag, Avi Tag (Cat. No.CDA-H82E9) captured on Biotin CAP- Series S Sensor Chip can bind Ritux*mab with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).

SPRのプロトコルを申し込む
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Immobilized Human FcRn

Fig. 9 Immobilized Cynomolgus / Rhesus macaque FcRn Protein (Cat. No. FCM-C5284) on CM5 Chip via anti-His antibody, can bind Herc*ptin with an affinity constant of 0.403 μM as determined in a SPR assay (Biacore T200).

SPRのプロトコルを申し込む
お問い合わせ
製品一覧
(全てのFc受容体はHEK293によって発現されている)
  • FcRn

  • FcγR

  • FcεR

分子名製品番号ホスト詳細情報構造
分子名製品番号ホスト詳細情報構造
分子名製品番号ホスト詳細情報構造

Fc受容体の関連分子-IgGFcタンパク質

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