NK Cell Immune Checkpoint Targets for Immunotherapy Research

Schematic of NK cell cytotoxicity types and functions against tumors

The purity of Human NKG2A&CD94, Fc Tag (Cat. No. NC4-H5257) is more than 95% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.

Immobilized Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-NKG2A (Z270), Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).

Immobilized Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) at 1 μg/mL (100 μL/well) can bind with a linear range of 0.2-8 ng/mL (QC tested).

Immobilized Human NKp46, His Tag (Cat. No. NC1-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-NKP46 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Immobilized PDGF-DD Protein, Human at 5 μg/mL (100 μL/well) can bind Human NKp44, Llama IgG2b Fc Tag (Cat. No. NC2-H5258) with a linear range of 0.008-0.25 μg/mL (Routinely tested).

Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Complex Protein (Cat. No. HLM-H82E4) captured on Biotin CAP-Series S Sensor Chip can bind Human LILRB1, His Tag (Cat. No. CDJ-H52H4) with an affinity constant of 10.8 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Biotinylated Human HLA-C*07:02:01 & B2M & RYR,His,Avitag™&Tag Free (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL2, Fc Tag (Cat. No. KI2-H5255) with an affinity constant of 1.01 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Loaded Biotinylated Human LILRA1, His,Avitag (Cat. No. LI1-H82E8) on SA Biosensor, can bind Human ANGPTL7, His Tag with an affinity constant of 0.204 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Flow cytometric analysis of HEK293/Human NKp46 Stable cells staining with PE-Labeled Monoclonal Anti-Human NKp46 Antibody, Mouse IgG1 (4E5) (Cat. No. FABm041-01) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

Expression analysis of human LILRB3 on HEK293/Human LILRB3 Stable Cell Line (Cat. No. CHEK-ATP159) by FACS. Cell surface staining was performed on HEK293/Human LILRB3 Stable Cell Line or negative control cell using PE-labeled anti-human LILRB3 antibody.

FACS analysis of NKp46 on HEK293/Human NKp46 Stable Cell Line (Cat. No. CHEK-ATP153). FACS assay shows that Biotinylated anti-NKP46, Human IgG1 | Human Kappa can bind to HEK293/Human NKp46 Stable Cell Line. HEK293/Human NKp46 Stable Cell Line was red line, Negative control HEK293 cells was grey line (QC tested).