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Biotinylated Human LAIR2 / CD306 Protein, His,Avitag™

  • Synonym
    LAIR2,CD306
  • Source
    Biotinylated Human LAIR-2, His,Avitag(LA2-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Pro 152 (Accession # Q6ISS4-1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    LAIR-2 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 17.8 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LAIR-2 SDS-PAGE

Biotinylated Human LAIR-2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 LAIR-2 ELISA

Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAIR-2, His,Avitag (Cat. No. LA2-H82E4) with a linear range of 2-78 ng/mL (QC tested).

  • Background
    Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) is also known as CD antigen CD306. LAIR2 contains one Ig-like C2-type (immunoglobulin-like) domain. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 / CD306 is thought to be secreted and may help modulate mucosal tolerance.
  • Clinical and Translational Updates

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