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Your Position: ホーム > 新世代の選択的蛍光標識技術プラットホームと製品

新世代の選択的蛍光標識技術プラットホームと製品

新世代の選択的蛍光標識技術プラットホームと製品
バックグラウンド
Star Staining Star Stainingは弊社の新世代の標識技術プラットホームによって開発された高品質の蛍光標識タンパク質です。タンパク質活性は影響されず、天然のコンフォメーションも保てます。ロット間の一貫性も良好です。我々は研究実力を持ち、優れている技術プラットホームを利用しStar Stainingを設計しました。 
Star StainingはCAR-T細胞の品質管理とサンプル解析のために利用できます。
フローサイトメトリーは細胞治療薬の開発プロセスにおいてCAR陽性T細胞を検出するための重要な技術的手段です。蛍光標識タンパク質はフルオレセインで事前に標識されているためワンステップで染色し、検出できます。プロセスが簡単で、短時間でできます。また二重抗体による非特異的なバックグラウンドを回避できるので、フローサイトメトリーによるCAR-T細胞の検出に最適だと考えられています。
蛍光標識技術は高品質の蛍光標識タンパク質を開発するためのキーポイントです。現在市場でよく使われている標識技術は通常のランダムの化学的方法であるため、蛍光の均一性を保証できないほか、タンパク質の活性部位は標識に覆われてしまい、タンパク質の活性が影響される可能性があります。そういった方法でロット間の均質性と生物活性の高い蛍光標識タンパク質は作れません。ある研究によると、新世代の蛍光標識技術を使うことで特異的な部位を標識でき、短時間で高効率で、またタンパク質の天然的コンフォメーションも影響されず、生物活性と検出の感度も高くて、ロット間の均質性が良好で、非特異的シングナルもないので、高品質の蛍光標識タンパク質の開発に最適だという。
技術の原理
通常の化学標識技術
Traditional Chemical Labeling
ランダムに標識するため、タンパク質の構造は影響される。
タンパク質の活性部位は覆われる。
タンパク質の親水性は変えられ、凝集を引き起こす。
ロット間のばらつきが多い
新世代の標識技術
New-generation Site-specific Labeling
タンパク質の特異的部位を標識できる。
標識部位は活性部位から離れているので、天然構造のコンフォメーションを維持できる。
生体直交反応の効率は高くて、非特異的なシグナルもない。
ロット間の均質性
Star Stainingの特長

弊社は細胞医薬品の品質管理と臨床サンプルの分析に適用する高品質の試験用試薬の開発に取り込んでおりまして、長年の経験を積んで技術改善をし、研究実力を持って高水準のStar Staining––新たな蛍光標識プラットホームを構築しました。Star Stainingによって製造された製品はユニークな特長を持っており、細胞医薬品の研究や開発に貢献できることが期待されます。

新世代の標識技術が使われ、高い生物活性が保てる。

高感度&高特異性はCAR細胞によって検証済みである。

非特異的シグナルが低く、二重の蛍光色素PBMCの分析済みである。

ロット間の均質性があり、臨床サンプルの分析や検査に最適。

人気商品一覧
蛍光タイプ分子製品番号詳細情報注文/事前注文
全star stainning製品リスト
  • FITC

  • PE

  • APC

  • Alexa Fluor 647

  • Alexa Fluor 555

  • Alexa Fluor 488

Fluorescent LabelsMoleculeProduct Description
FITCBCMAFITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19FITC-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinFITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3FITC-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
HER2FITC-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
CD7FITC-Labeled Human CD7 Protein, His Tag star staining
Siglec-3FITC-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
CD30FITC-Labeled Human CD30 / TNFRSF8 Protein, His Tag star staining
EGF RFITC-Labeled Human EGF R Protein, His Tag star staining
EGFRVIIIFITC-Labeled Human EGFRvIII Protein, His Tag star staining
GUCY2CFITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His Tag star staining
NKG2DFITC-Labeled Human NKG2D / CD314 Protein, His Tag star staining
FAPFITC-Labeled Human FAP Protein, His Tag star staining
Nectin-4FITC-Labeled Human Nectin-4 Protein, His Tag star staining
CD37FITC-Labeled Human CD37 Protein, His Tag star staining
protein LFITC-Labeled Recombinant Protein L Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
PEBCMAPE-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19PE-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinPE-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3PE-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
NKG2DPE-Labeled Human NKG2D / CD314 Protein, His Tag star staining
FAPPE-Labeled Human FAP Protein, His Tag star staining
Nectin-4PE-Labeled Human Nectin-4 Protein, His Tag star staining
CD37PE-Labeled Human CD37 Protein,  His Tag star staining
protein LPE-Labeled Recombinant Protein L Protein, His Tag star staining
EMMPRINPE-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300ePE-Labeled Human CD300e Protein, His Tag star staining
HER2PE-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2PE-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3PE-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1PE-Labeled Human PD-1 Protein, His Tag star staining
OX40PE-Labeled Human OX40 Protein, His Tag star staining
Mucin-1PE-Labeled Human MUC-1 Protein, His Tag star staining
TSLP RPE-Labeled Human TSLP R Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
APCBCMAAPC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19APC-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAPC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3APC-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
EMMPRINAPC-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300eAPC-Labeled Human CD300e Protein, His Tag star staining
HER2APC-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2APC-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3APC-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1APC-Labeled Human PD-1 Protein, His Tag star staining
OX40APC-Labeled Human OX40 Protein, His Tag star staining
Mucin-1APC-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining
TSLP RAPC-Labeled Human TSLP R Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
Alexa Fluor 647BCMAAlexa Fluor 647-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19Alexa Fluor 647-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAlexa Fluor 647-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3Alexa Fluor 647-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
EMMPRINAlexa Fluor 647-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300eAlexa Fluor 647-Labeled Human CD300e Protein, His Tag star staining
HER2Alexa Fluor 647-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2Alexa Fluor 647-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3Alexa Fluor 647-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1Alexa Fluor 647-Labeled Human PD-1 Protein, His Tag star staining
OX40Alexa Fluor 647-Labeled Human OX40 Protein, His Tag star staining
Mucin-1Alexa Fluor 647-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining
TSLP RAlexa Fluor 647-Labeled Human TSLP R Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
Alexa Fluor 555BCMAAlexa Fluor 555-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19Alexa Fluor 555-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAlexa Fluor 555-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3Alexa Fluor 555-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
NKG2DAlexa Fluor 555-Labeled Human NKG2D / CD314 Protein, His Tag star staining
FAPAlexa Fluor 555-Labeled Human FAP Protein, His Tag star staining
Nectin-4Alexa Fluor 555-Labeled Human Nectin-4 Protein, His Tag star staining
CD37Alexa Fluor 555-Labeled Human CD37 Protein,  His Tag star staining
protein LAlexa Fluor 555-Labeled Recombinant Protein L Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
Alexa Fluor 488BCMAAlexa Fluor 488-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19Alexa Fluor 488-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAlexa Fluor 488-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3Alexa Fluor 488-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
EMMPRINAlexa Fluor 488-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300eAlexa Fluor 488-Labeled Human CD300e Protein, His Tag star staining
HER2Alexa Fluor 488-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2Alexa Fluor 488-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3Alexa Fluor 488-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1Alexa Fluor 488-Labeled Human PD-1 Protein, His Tag star staining
OX40Alexa Fluor 488-Labeled Human OX40 Protein, His Tag star staining
Mucin-1Alexa Fluor 488-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining
TSLP RAlexa Fluor 488-Labeled Human TSLP R Protein, His Tag star staining
* Some products are still under development. If you have any requirements, please click here to contact us.
検証データ

高純度

90%以上の高純度のStar Staining FITC-labeled Human BCMA
BCMA SDS-PAGE

FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

BCMA SEC-MALS

The purity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

高い生物活性

他社の製品より高い結合活性
BCMA binding activity verified by FACS
CD19 binding activity verified by FACS

Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.

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Alexa Fluor 647-Labeled Human Mesothelin (296-580)

The activity of Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) was higher than other Competitor.


Alexa Fluor 488-Labeled Human Mesothelin / MSLN (296-580) Protein

The activity of Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) was higher than other Competitor.

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Star Staining PE-CD19またはAPC-CD19タンパク質は、フローサイトメトリーにより、CD19CARの発現を高感度で検出できます。
APC-CD19 FACS binding activity verified by FACS

Anti-CD19 CAR-293 cells were stained with PE-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HP2H5), His Tag star staining and negative control protein respectively, PE signal was used to evaluate the binding activity.

PE-CD19 binding activity verified by FACS

Anti-CD19 CAR-293 cells were stained with APC-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HA2H9), His Tag star staining and negative control protein respectively, APC signal was used to evaluate the binding activity.

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非還元性PBMCへの非特異的結合

Star Staining製品は、非還元PBMCに対する非特異的結合がなく、他社製品と比較して高い結合活性を持っています。
BCMA-PBMC  verified by FACS

Non-specific binding to non-transduced PBMCs between PE-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with PE -Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and PE signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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BCMA PBMC comparison Data

Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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自然な生物活性を保てます

FACSとSPRによって検証された、結合前/後の高い結合能力
BCMA bioactivity verified by FACS

Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.

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BCMA binding affinity  verified by SPR
FITC-BCMA binding affinity  verified by SPR

Binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag have almost the same level of affinity.

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ロット間の一貫性が高い

異なるロットのFITC/Alexa Fluor-555/PE標識ヒトBCMAタンパク質の結合活性がFACSによって確認されています。
FITC-BCMA batch-to-batch consistency verified by FACS
AF555-BCMA  batch-to-batch consistency verified by FACS
PE-BCMA batch-to-batch consistency verified by FACS

Binding activity of different lots of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) , AF555-Labeled Human BCMA (Cat. No. BCA-HA2H6) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.

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良好な安定性

PE-BCMA stability verified by FACS
FITC-BCMA stability verified by FACS

PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) and FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3)  25℃ for 48 hours and freeze-throw cycles are stable without performance reduction.

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CAR-Tについてはこちら

参考文献

[1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print, 2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.

[2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.

[3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.

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