Leave message
Can’t find what you’re looking for?
Fill out this form to inquire about our customタンパク質 services!
カスタムサービスについてのお問い合わせはこちら >>
Offering SPR-BLI Services - Proteins provided for free! Here come GMP Grade Cytokines！Free Sample is available！
Fill out organ-on-a-chip questionnaire to win a FREE gift! 
Fill out organ-on-a-chip questionnaire to win a FREE gift!
Thank you for choosing ACROBiosystems. Would you rate our product and service？
A-Z
> 磁性粒子化学発光免疫測定法（MPCLIA）構築のための試薬
磁性粒子（MP）化学発光免疫測定法（CLIA）は、磁気ビーズの柔軟性とCLIAの感度と特異性を組み合わせた分析法です。ウェルプレートにタンパク質や抗体を固定化する代わりに、磁気ビーズを使用することで、サンプル接触表面積、捕捉効率、自動化機能を大幅に向上させることができます。そのため、MPCLIAは疾病診断や化学反応のモニタリングなど、多くの研究分野で頻繁に利用されます。
MPCLIAが従来のELISAと最も異なる点は、足場となる磁気ビーズに加え、化学発光による検出を行うことです。化学反応を利用してホースラディッシュ ペルオキシダーゼ (HRP) やアクリジニウム エステル (AE) などの物質を励起することにより、光子を放出させ、専用の化学発光リーダーで測定することができます。CLIA法は、従来のELISA法に比べ、感度、特異性、直線性において優れていることが確認されています。
Chemiluminescence Detection Mechanism
Colorimetric (ELISA) Detection Mechanism
| 製品名 | 製品番号 | 詳細 | 予約/注文する |
|---|
| Assay No. | 磁気ビーズ | サンプル１ | サンプル２ | マーカー | 基質溶液 |
|---|---|---|---|---|---|
| 1 | Protein A-coupled Magnetic Beads (Cat. No. MPC-A001) | Human Antibody | Biotinylated non-Fc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | Substrate Solution(AE Marker) (Cat. No. ABK-001) |
| 2 | hFc tag protein | ||||
| 3 | Protein G-coupled Magnetic Beads (Cat. No. MPC-A002) | Human/Mouse Antibody | Biotinylated non-Fc tag protein | ||
| 4 | Fc tag protein | ||||
| 5 | Anti-His-coupled Magnetic Beads (Cat. No. MPC-A005) | His tag protein | Biotinylated non-His tag protein | ||
| 6 | Human antibody | Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) | |||
| 7 | hFc tag protein | ||||
| 8 | Anti-Mouse IgG-coupled Magnetic Beads (Cat. No. MPC-A003) | Mouse antibody | Biotinylated non-mFc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | |
| 9 | mFc tag protein | ||||
| 10* | Mouse antibody | hFc tag protein | Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) | ||
| 11* | mFc tag protein | Human antibody | |||
| 12* | hFc tag protein | ||||
| 13 | Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) | Biotinylated non-hFc tag protein | Human antibody | ||
| 14 | hFc tag protein | ||||
| 15 | Biotinylated non-mFc tag protein | Mouse antibody | Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S163) | ||
| 16 | mFc tag protein | ||||
| 17 | Anti-Human IgG-coupled Magnetic Beads (Cat. No. MPC-A004) | Human antibody | mFc tag protein | ||
| 18 | hFc tag protein | ||||
| 19* | hFc tag protein | Mouse antibody | |||
| 20* | Human antibody | Biotinylated non-hFc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | ||
| 21* | hFc tag protein |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetic Particle Chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
| Cat. No. | Product Description |
|---|---|
| PD1-H82E4 | Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified) |
| STN-NA114 | Streptavidin Protein-Acridine ester (MALS verified) |
| PD1-H5258 | Human PD-L1 / B7-H1 Protein, Fc Tag (MALS verified) |
| MPC-A001 | Protein A-coupled Magnetic Beads (recommended for MPCLIA) |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S163, 0.04 μg /Test). Detection was performed with sensitivity of 1.5625 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
| Cat. No. | Product Description |
|---|---|
| PD1-H82E4 | Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified) |
| MPC-A006 | Streptavidin-Magnetic Beads (recommended for MPCLIA) |
| PD1-H52A3 | Human PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified) |
| AMG-S163 | Anti-Mouse IgG-Acridine ester (MALS verified) |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Anti-Human IgG Antibody-Acridine ester (Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
When used the Chemiluminescent Substrate Solution (AE Marker) (Cat. No. ABK-001) in a sandwich MPCLIA Assay for detection of SARS-CoV-2, The Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-BM266) coupled Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) was incubated with Acridine Ester-Labeled Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM224) (Cat. No. NUN-M224) and treated with SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at increasing concentration, detection was performed using with sensitivity of 97.7 pg/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
When used the Chemiluminescent Substrate Solution (HRP Marker) (Cat. No. ABK-002) in a sandwich CLEIA Assay for detection of Human IFN-gamma, immobilized Monoclonal Anti-IFNγ antibody, Human IgG1 at 2 μg/mL, add increasing concentrations of Human IFN-gamma, premium grade (Cat. No. IFG-H4211) and then add Biotinylated Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG at 0.05 μg/mL. Detection was performed using Streptavidin-HRP, Horseradish peroxidase conjugated Streptavidin (Cat. No. STN-NH913) with sensitivity of 7.8 pg/mL.
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
The MPCLIA assay shows that Protein G-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A002) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-Human IgG-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A004) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-His Tag-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A005) is stable at 37°C for 10 days.
This web search service is supported by Google Inc.
アクロバイオシステムズの公式アカウント
