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Streptavidin(SA)製品群

Streptavidin(SA)製品群
SA- ビオチン系
1963 年、Stapley EO は、Streptomyces avidinii の培養液から初めてストレプトアビジン (SA) を分離しました。 SA とビオチン間の結合は、自然界で知られている最も強力な非共有相互作用の 1 つです。 SA とビオチンは高い親和性で結合し、大きな増幅効果をもたらすため、SA-ビオチン系は生物学および化学実験において広く活用されています。
SA-ビオチン系は多くの場合、抗体またはタンパク質に結合されます。SAをHRP(Horseradish Peroxidase)、蛍光色素などの標識に、ビオチンを抗体やタンパク質に結合させたもの (MABSOL® Biotinylated Proteins) が多く使用されています。
SA-ビオチンシステムは、微量抗原、抗体の定性的/定量的検出、局在観測研究などに広く使用されており、標的の間接検出を実現し、免疫標識やトレーサー分析をより高感度にします。その中でも、標識SAタンパク質は、プレミアムグレードの PE標識SA タンパク質を含んでおり、厳密に無菌であり、前臨床および臨床での細胞関連実験に使用することができます。
SA製品群
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検証データ

PE標識SAタンパク質のアプリケーションデータ(FACS)

Application data for PE labeled SA protein(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Application data for PE labeled SA protein(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

SAのアプリケーションデータ(ELISA)

STN-N5116-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

STN-N5116-E2

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

SA-HRPのアプリケーションデータ

Application data for SA-HRP

Immobilized Cynomolgus / Rhesus macaque BCMA, His Tag (Cat. No. BCA-C52H7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 0.5-16 ng/mL when detected by Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NH913) (QC tested).

Application data for SA-HRP

Measure performance comparison between Acro (Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin) and other competitive products. With tight quality control measures, Acro has better sensitivity than other brands.

SAコーティングプレートのアプリケーションデータ

SP-11-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

SP-11-E2

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

SP-11-E3

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituxima* with a linear range of 78-625 ng/mL (QC tested).

SP-11-E4

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimuma* with a linear range of 78-1250 ng/mL (QC tested).

SA磁気ビーズのアプリケーションデータ

SMB-B01-E1

Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads (Cat. No. SMB-B01) can bind the Anti-SARS-CoV-S protein RBD Antibody with an EC50 1.157 μg/mL (QC tested).

MABSOL® ビオチン化蛋白質

Biotinylated Protein

リファレンス

  • Louis Chaiet, Frank J. Wolf. The properties of streptavidin, a biotin-binding protein produced by Streptomycetes, Archives of Biochemistry and Biophysics (1964). https://doi.org/10.1016/0003-9861(64)90150-X.

ACRO Quality

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