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免疫チェックポイント タンパク質

Immune Checkpoint Proteins
バックグラウンド

免疫チェックポイント(immune checkpoint)は免疫システムにおける重要な調節因子です。免疫チェックポイントを用いて開発されている抗体医薬品は重要な免疫療法剤として、がん、がん、免疫疾患などの治療に使われています。CTLA-4とPD-1/PD-L1は現在最も研究されている抑制受容体で、免疫チェックポイントを抑制し、がんを治療します。LAG-3、TIM-3、TIGIT、VISTA、B7-H3などの免疫チェックポイント受容体抗体薬の開発が研究で進んでいます。また、GITR、4-1BB、CD40などの活性化受容体を使った分子標的薬の研究も始められています。このように、免疫チェックポイント抗体薬の開発を効率的に行うことが非常に重要です。

免疫チェックポイントタンパク質が適用されている医薬品創薬をご確認ください。

免疫チェックポイントの経路
CD155 ICOSL CD86 CD80 MHC CD40 PD-L2 PD-L1 4-1BBL OX40L GITRL MHC PD-L1 Galectin 9 TIGIT ICOS CTLA-4 CD28 TCR CD40L PD-1 PD-1 4-1BB OX40 GITR TCR PD-1 TIM-3 TCR PD-1 PD-1 LAG-3 MHC PD-L1 PD-L2 MHC-LL Dendritic cell Tumor cell Macrophage T-cells

分子名をクリックし詳細情報をご確認ください。

Stimulatory signal

Inhibitory signal

ほかの免疫チェックポイントタンパク質についてはこちら

製品特長&用途
製品の特徴:
高い純度がMALS/HPLCの検証によって、高い生物活性はELISA/SPR/FACSなどの検証によって確認された。厳しいQCによって、高いバッチ間一貫性を保証できます。
製品の用途
製品リスト

分子名をクリックし詳しい情報を確認

  • 免疫共抑制分子

  • 免疫共刺激分子

  • ほかの免疫チェックポイント分子一覧

  • 阻害剤スクリーニングキット製品リスト

分子名種族タグ標識検証データ
(プロトコルを提供可能)
PD-1Human, Mouse, Canine, Cynomolgus, Rabbit, Rat, Rhesus macaqueFc, His, Fc & His, Fc & Avi & His, Fc & Avi, His & Avi, mFc& Avi, Twin-Strep, Llama Fc , mFcBiotin-labeled, PE-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, SPR, FACS
PD-L1Human, Mouse, Rabbit, Canine, Cynomolgus / Rhesus macaqueFc, His, Fc & Avi, His & Avi, Fc & Avi & His, mFc, LlamaFc, mFc& Avi, Twin-StrepBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, SPR, FACS
PD-L2Human, MouseFc, His, Fc & Avi, Avi & His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, SPR, BLI, FACS, Cell based assay
B7-H3Human, Mouse, CynomolgusFc & Avi, Avi & His, His, Fc, Avi & HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, Cell based assay
B7-H4Human, MouseHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
B7-H5Human, Mouse, Rhesus macaqueFc & Avi, Avi & His, His, Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI
BTLAHuman, MouseFc & Avi, His & Avi, His, mFc, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
CD80(B7-1)Human, Mouse, Cynomolgus / Rhesus macaqueFc, Fc & Avi, Avi & His, mFc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
CD86(B7-2)Human, Mouse, Cynomolgus / Rhesus macaqueFc & Avi, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
CD96HumanHis & Avi, His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD47Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, CanineFc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFcBiotin-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
CD155Human, Mouse, Rhesus macaqueFc & Avi, His & Avi, mFc, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CTLA-4Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaqueFc, His, Fc & Avi, His & Avi, mFc, mFc& AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
LAG-3Human, Mouse, Rat, Cynomolgus, MarmosetFc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
TIGITHuman, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, CaninemFc& Avi, Fc & Avi, His & Avi, His, Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI
TIM-3Human, Mouse, CynomolgusAvi & His, mFc, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR
CD111HumanHis, FcUnconjugatedSDS-PAGE, ELISA, BLI
DNAM-1Human, CynomolgusFc & Avi, His, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA, BLI, FACS
Galectin-9HumanHis, MBP & HisUnconjugatedSDS-PAGE
HVEMHumanHis, Twin-Strep, Fc, mFc, Fc & AviBiotin-labeled, Unconjugated ,SDS-PAGE, ELISA, BLI
Nectin-2HumanHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, BLI
Nectin-3HumanHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
PVRIGHuman, Mouse, CynomolgusFc & Avi, mFc& Avi, His & Avi, His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
SIRP alphaHuman, Mouse, CynomolgusFc, His & Avi, mFc& Avi, mFc, HisBiotin-labeled, PE-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
SIRP alpha V2HumanHisBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
SIRP gammaHumanFcUnconjugatedSDS-PAGE, ELISA
CD160HumanHisUnconjugatedSDS-PAGE, ELISA
分子名種族タグ標識検証データ
(プロトコルを提供可能)
4-1BB LigandHuman, Mouse, Rat, CynomolgusFc, Fc & Avi, mFc, His & FlagBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, Cell based assay
4-1BBHuman, Mouse, Rat, Rabbit, Cynomolgus / Rhesus macaque, CanineHis, Fc, Fc & Avi, His & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR
OX40 LigandHuman, Mouse, Rhesus macaqueFc, His, His & Avi, Avi & Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
OX40Human, Mouse, Rabbit, Rat, Rhesus macaque, MarmosetHis, Fc, His & Avi, Fc & Avi, Avi & His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
ICOS Ligand
(B7-H2)
Human, Mouse, CynomolgusHis, FcUnconjugatedSDS-PAGE, ELISA
ICOSHuman, Mouse, CynomolgusHis, Fc, His & AviBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
GITR LigandHuman, MouseHis, Fc, Avi & FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
GITRHuman, Mouse, Rat, Rhesus macaque, CanineHis, Fc, His & Avi, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA
CD48HumanHis, Fc, mFcUnconjugatedSDS-PAGE, ELISA
HVEMHumanHis, Fc, Fc & Avi, mFc, TwiStrepBiotin-labeled, UnconjugatedSDS-PAGE, MALS, BLI
Nectin-2HumanHis, Fc, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI
CD40 LigandHuman, Mouse, Human / Rhesus macaqueHis, Fc, His & Avi, Avi & Fc, Avi & His & Flag, His & Flag, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
CD40Human, Mouse, Rabbit, Cynomolgus, Rhesus macaqueHis, Fc, Avi & His, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD30 ligandHumanHis, mFcUnconjugatedSDS-PAGE, ELISA, BLI, FACS
CD30HumanHis, Fc, Avi & His, LlamaFcBiotin-labeled, PE-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD28Mouse, Human / Cynomolgus / Rhesus macaqueHis, Fc, His & Avi, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, SPR, BLI
CD27 LigandHuman, MouseHis & Avi, mFc, His, His & Avi & Flag, His & Flag, FcBiotin-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA
CD27Human, MouseHis, Fc, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA
B7-1Human, Mouse, Cynomolgus / Rhesus macaqueFc, Fc & Avi, Avi & His, mFc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
B7-2Human, Mouse, Cynomolgus / Rhesus macaqueHis, Fc, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
LIGHTHumanHis, mFc, FcUnconjugatedSDS-PAGE, MALS, ELISA, BLI
B7-H7Human, CynomolgusHis, Fc, His & Avi, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, BLI
B7-H6Human, CynomolgusHis, His & Avi, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, ELISA, SPR
2B4HumanHis, Fc, Avi & HisBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
CD28HHumanHis, FcUnconjugatedSDS-PAGE, ELISA
NKp30HumanHis, FcUnconjugatedSDS-PAGE, SPR
製品番号詳細情報Detection mechanism予約注文/注文
実験データ

独自の実験方法を無償で提供しております。製品のページから関連のプロトコルを申し込んでください。

真の分子量と構造はMALSで検証済みである。

CD27 Ligand, MALS-validated protein

Figure 1. The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) is more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.

ロット間の一貫性は良好である

組換えタンパク質の純度と生物活性の安定性はとても重要です。弊社が作っている全てのタンパク質の純度と生物活性は様々な検証によって確認されています。

PD-1, MALS-validated protein

Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)(Cat. No. PD1-H82E4

1免疫/抗体のスクリーニングに利用できる

抗体の結合を検証

PD-1&PD-L1 pathway

PD-1, MALS-validated protein1

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind anti-PD-1 mab with a linear range of 0.1-3 ng/mL (Routinely tested).                  

PD-1&PD-L1 pathway

PD-1, MALS-validated protein2

Serial dilutions of anti-PD-1 mab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

弊社はPD1: PD-L1(Cat. No. EP-101)とCD47: SIRP alpha(Cat. No. EP-101) and CD47: SIRP alpha (Cat. No. EP-102)の阻害剤スクリーニングキットも開発しています。

阻害剤スクリーニングキットについてはこちら

抗体医薬品の親和性アッセイ

LAG-3 pathway

LAG-3, BLI-validated protein

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

LAG-3 pathway

LAG-3, SPR-validated protein

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

中和抗体の検証

TIGIT pathway

TIGIT, MALS-validated protein

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

CD47&SIPR α pathway(Cell-based assay)

CD47, FACS-validated protein

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL

2細胞機能の検証

4-1BB pathway

4-1BB Ligand, Cell-based assay validated protein

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).


3薬物動態分析−PK分析

CTLA-4 pathway

CTLA-4 dimer, Cell-based assay validated protein

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No. CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag, His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

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