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Biotinylated Human BTN1A1 / Butyrophilin Protein, His,Avitag™

  • シノニム
    BTN1A1,Butyrophilin,BTN
  • ソース
    Biotinylated Human BTN1A1, His,Avitag (BT1-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 242 (Accession # Q13410-1).
    Predicted N-terminus: Ala 27
  • 分子特性
    Online(Ala 27 - Arg 242) Q13410-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 27.6 kDa. The protein migrates as 36-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >90% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human BTN1A1, His,Avitag (Cat. No. BT1-H82E6) SDS-PAGE gel

Biotinylated Human BTN1A1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • バックグラウンド
    Butyrophilin subfamily 1 member A1 (BTN1A1) is also known as BTN, which is a member of the immunoglobulin superfamily and the major protein associated with fat droplets in the milk. BTN1A1 may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families. Furthermore, BTN1A1 regulates the amount of lipids and size of droplets expressed in milk and inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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