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Human BCMA / TNFRSF17 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • シノニム
    TNFRSF17,CD269,BCM,BCMA
  • ソース
    Human BCMA, Mouse IgG2a Fc Tag (BCA-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
    Predicted N-terminus: Met 1
  • 分子特性
    Online(Met 1 - Ala 54) Q02223-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 32.8 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • エンドトキシン
    Less than 0.1 EU per μg by the LAL method.
  • 純度

    >90% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) SDS-PAGE gel

Human BCMA, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human BCMA, Mouse IgG2a Fc TagHuman BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) ELISA bioactivity

Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 1 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.2-6 ng/mL (QC tested).

Human BCMA, Mouse IgG2a Fc TagHuman BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) ELISA bioactivity

Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA MAb with a linear range of 0.2-3 ng/mL (Routinely tested).

Human BCMA, Mouse IgG2a Fc TagHuman BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) ELISA bioactivity

Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) with a linear range of 0.2-2 ng/mL (Routinely tested).

  • バックグラウンド
    Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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