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Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

  • シノニム
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • ソース
    Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (CDG-C5257) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1(CD3E) & Q95LI7-1(CD3G)).
    Predicted N-terminus: Gln 22 (CD3E) & Gln 23 (CD3G)
  • 分子特性
    Online(Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)) Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)

    Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 40.6 kDa (CD3E) and 40.4 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 45-52 kDa due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 成分

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5257) SDS-PAGE gel

Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ) MALS images

The purity of Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5257) is more than 90% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc TagCynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5257) ELISA bioactivity

Immobilized Bispecific T cell Engager (CD3 X BCMA) at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag(Cat. No. CDG-C5257) with a linear range of 0.3-5 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5257) on AHC Biosensor, can bind Human CD3×BCMA scFV with an affinity constant of 1.02 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • バックグラウンド
    T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

価格(JPY) : 98,000

価格(JPY) : 711,200

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