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Your Position: ホーム > Protein > Integrin isoform alpha-7X2B beta 1 > IT1-H82W5

Biotinylated Human Integrin isoform alpha-7X2B beta 1 (ITGA7X2B&ITGB1) Heterodimer Protein, Tag Free&His,Avitag™

  • シノニム
    Integrin isoform alpha-7X2B beta 1,ITGA7X2BB1,ITGA7X2B&ITGB1
  • ソース
    Biotinylated Human ITGA7X2B&ITGB1 Heterodimer Protein, Tag Free&His,Avitag (IT1-H82W5) is expressed from human 293 cells (HEK293). It contains AA Phe 34 - Trp 1038 (ITGA7X2B) & Gln 21 - Asp 728 (ITGB1) (Accession # Q13683-7(ITGA7X2B) & P05556-1(ITGB1)).
    Predicted N-terminus: Phe 34 (ITGA7X2B) & Gln 21 (ITGB1)
  • 分子特性
    Online(Phe 34 - Trp 1038 (ITGA7X2B) & Gln 21 - Asp 728 (ITGB1)) Q13683-7 (ITGA7X2B) & P05556-1 (ITGB1)

    Biotinylated Human ITGA7X2B & ITGB1 Heterodimer Protein, produced by co-expression of ITGA7X2B and ITGB1, has a calculated MW of 116.9 kDa (ITGA7X2B) and 86.7 kDa (ITGB1). Subunit ITGA7X2B is fused with an acidic tail but no tag at the C-terminus and subunit ITGB1 contains a basic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™). The non-reducing (NR) protein migrates as 100-115 kDa due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >90% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ITGA7X2B&ITGB1 Heterodimer Protein, Tag Free&His,Avitag (Cat. No. IT1-H82W5) SDS-PAGE gel

Biotinylated Human ITGA7X2B&ITGB1 Heterodimer Protein, Tag Free&His,Avitag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • バックグラウンド
    Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers, which is highly expressed in cardiac muscle, skeletal muscle and smooth muscle cells, and localizes to Z-disc and costamere structures. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). ITGA7 has been shown to interact with Merosin, ITGB1, FHL2 and FHL3.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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