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Your Position: ホーム > Protein > IL-31 > IL1-M82Q9

Biotinylated Mouse IL-31 Protein, His,Avitag™ (MALS verified)

  • シノニム
    IL-31,IL31,Interleukin-31
  • ソース
    Biotinylated Mouse IL-31, His,Avitag (IL1-M82Q9) is expressed from human 293 cells (HEK293). It contains AA Thr 24 - Cys 163 (Accession # Q6EAL8-1).
    Predicted N-terminus: His
  • 分子特性
    Online(Thr 24 - Cys 163) Q6EAL8-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 19.2 kDa. The protein migrates as 20-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse IL-31, His,Avitag (Cat. No. IL1-M82Q9) SDS-PAGE gel

Biotinylated Mouse IL-31, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Mouse IL-31, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse IL-31, His,Avitag (Cat. No. IL1-M82Q9) is more than 90% and the molecular weight of this protein is around 22-34kDa verified by SEC-MALS.

  • バックグラウンド
    IL-31 is an inflammatory cytokine that helps trigger cell-mediated immunity against pathogens. Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. It has also been identified as a major player in a number of chronic inflammatory diseases, including atopic dermatitis. May function in skin immunity. IL-31 is produced by a variety of cells, namely type 2 helper (TH2) T-cells. IL-31 sends signals through a receptor complex made of IL-31RA and oncostatin M receptor β (OSMRβ) expressed in immune and epithelial cells. These signals activate three pathways: ERK1/2 MAP kinase, PI3K/AKT, and JAK1/2 signaling pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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