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Your Position: ホーム > Protein > IL-33 > IL3-M82Q7

Biotinylated Mouse IL-33 Protein, His,Avitag™ (MALS verified)

  • シノニム
    IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2
  • ソース
    Biotinylated Mouse IL-33, His,Avitag (IL3-M82Q7) is expressed from human 293 cells (HEK293). It contains AA Ser 109 - Ile 266 (Accession # Q8BVZ5-1).
  • 分子特性
    Online(Ser 109 - Ile 266) Q8BVZ5-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, 1 mM TCEP, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse IL-33, His,Avitag (Cat. No. IL3-M82Q7) SDS-PAGE gel

Biotinylated Mouse IL-33, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Mouse IL-33, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse IL-33, His,Avitag (Cat. No. IL3-M82Q7) is more than 90% and the molecular weight of this protein is around 20-26 kDa verified by SEC-MALS.

  • バックグラウンド
    Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2,and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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