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Your Position: ホーム > Protein > TNF-beta > TNB-H52H3

Human TNF-beta / Lymphotoxin-alpha Protein, His Tag (MALS verified)

  • Synonym
    LTA,DAMA-25N12,13-004,LT,TNFB,TNFSF1,LT-alpha,TNF-beta
  • Source
    Human TNF-beta Protein, His Tag(TNB-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 35 - Leu 205 (Accession # P01374).
    Predicted N-terminus: Leu 35
  • Molecular Characterization
    TNF-beta Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 20.5 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TNF-beta SDS-PAGE

Human TNF-beta Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
TNF-beta MALS images

The purity of Human TNF-beta Protein, His Tag (Cat. No. TNB-H52H3) is more than 90% and the molecular weight of this protein is around 62-78 kDa verified by SEC-MALS.

Bioactivity-ELISA
 TNF-beta ELISA

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag (Cat. No. TNB-H52H3) with a linear range of 1-20 ng/mL (QC tested).

  • Background
    The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.
  • Clinical and Translational Updates

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