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Biotinylated Human LY6G6D Protein, His,Avitag™

  • Synonym
    lymphocyte antigen 6 family member G6D,C6orf23,G6D,LY6-D,MEGT1,NG25
  • Source
    Biotinylated Human LY6G6D Protein, His,Avitag(LYD-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Ser 104 (Accession # O95868-1).
  • Molecular Characterization
    LY6G6D Structure

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 12.8 kDa. The protein migrates as 17-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LY6G6D SDS-PAGE

Biotinylated Human LY6G6D Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 LY6G6D ELISA

Immobilized Biotinylated Human LY6G6D Protein, His,Avitag (Cat. No. LYD-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-LY6G6D antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction.
  • Clinical and Translational Updates

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