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Your Position: ホーム > Protein > MICB > MIB-H52H3

Human MICB Protein, His Tag (MALS verified)

  • Synonym
    PERB11.2
  • Source
    Human MICB Protein, His Tag(MIB-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Thr 308 (Accession # NP_005922.2).
    Predicted N-terminus: Ala 23
  • Molecular Characterization
    MICB Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 34.5 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
MICB SDS-PAGE

Human MICB Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
MICB MALS images

The purity of Human MICB Protein, His Tag (Cat. No. MIB-H52H3) is more than 85% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
 MICB ELISA

Immobilized Human NKG2D Protein, Fc Tag (Cat. No. NKD-H5265) at 5 μg/mL (100 μL/well) can bind Human MICB Protein, His Tag (Cat. No. MIB-H52H3) with a linear range of 0.039-0.625 μg/mL (QC tested).

 MICB ELISA

Immobilized Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) at 5 μg/mL (100 μL/well) can bind Human MICB Protein, His Tag (Cat. No. MIB-H52H3) with a linear range of 0.039-0.313 μg/mL (Routinely tested).

  • Background
    Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
  • Clinical and Translational Updates

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