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ClinMax™ Human Granzyme B ELISA Kit

For research use only.
Product Details
Assay TypeSandwich-ELISA
AnalyteGranzyme B
Format96T
ReactivityHuman
Sensitivity1 pg/mL
Standard Curve Range3.906 pg/mL-500 pg/mL
Assay Time2 hr
Suitable Sample TypeFor the quantitative determination of humanGranzyme B in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Materials Provided
IDComponentsSize
CEA033-C01Pre-coated Anti- Granzyme B Antibody Microplate1 plate
CEA033-C02Human Granzyme B Standard20 μL
CEA033-C03Biotin-Anti- Granzyme B Antibody Con. Solution100 μL
CEA033-C04Biotin-Antibody Dilution Buffer8 mL
CEA033-C05Streptavidin-HRP Con. Solution0.5 mL
CEA033-C06Streptavidin-HRP Dilution Buffer15 mL
CEA033-C0720× Washing Buffer50 mL
CEA033-C08Sample Dilution Buffer15 mL ×2
CEA033-C09Substrate Solution12 mL
CEA033-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human Granzyme B that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human Granzyme B in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     Granzyme B Assay Principles
Typical Data Please refer to DS document for the assay protocol.
 Granzyme B TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of Granzyme B is less than 1 pg/mL.

  • Background: Granzyme B
    Granzyme B (GrB) is one of the serine protease granzymes most commonly found in the granules of natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells.
  • Clinical and Translational Updates

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