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Your Position: ホーム > Protein > T4 RNA Ligase 1 > T41-E5143

T4 RNA Ligase 1(10U/ul)

  • Product Details
    T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi.
  • Application
    1. Joining RNA to RNA
    2. Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
    3. Specific modifications of tRNAs
    4. RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
    5. Site-specific generation of composite primers for PCR
  • Unit Definition
    One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C.
  • Quility Control
    No Host Cell Protein and DNases residues.
  • Purity
    >90% as determined by SDS-PAGE.
  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM Tris, 0.1 mM EDTA, 1 mM DTT, pH 7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. This product is stable for up to 12 months at -20℃ from date of receipt.
  • Notes

    The coexistence of PEG promotes the response without changing the specificity of the reaction.

SDS-PAGE
T4 RNA Ligase 1 SDS-PAGE

T4 RNA Ligase 1(10U/ul) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 19,040

価格(JPY) : 83,720

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