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HEK293/Human NAPI-IIb Stable Cell Line

  • Description
    The HEK293/Human NAPI-IIb Stable Cell Line was engineered to express the receptor full length human NAPI-IIb (Gene ID: 10568), used to mimic cancer target cells. Surface expression of Human NAPI-IIb was confirmed by flow cytometry.
  • Application

    • Useful for cell-based NAPI-IIb binding assay

  • Growth Properties
    Adherent
  • Selection Marker
    Puromycin (2 μg/mL)
  • Culture Medium
    DMEM + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Bioactivity
 NAPI-IIb FACS

Expression analysis of human NAPI-IIb on HEK293/Human NAPI-IIb Stable Cell Line by FACS.
HEK293/Human NAPI-IIb Stable Cell Line or negative control cell was fixed with 4% paraformaldehyde followed by staining with PE-labeled anti-human NAPI-IIb antibody.

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  • Background
    NAPI-IIb, NaPi-2b, Npt2b, or SLC34A2 enables sodium:phosphate symporter activity. Acts upstream of or within in utero embryonic development and phosphate ion transport. Located in apical plasma membrane and brush border. Is expressed in several structures, including egg cylinder; genitourinary system; small intestine; and tooth. Human ortholog(s) of this gene implicated in pulmonary alveolar microlithiasis. Orthologous to human SLC34A2 (solute carrier family 34 member 2). [provided by Alliance of Genome Resources, Apr 2022]
  • License Disclosure
    This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 980,000

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