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SARS-CoV-2 Spike S1 (B.1.1.529) Specific ELISA Kit (For Vaccine Development)

For research use only.
IDComponentsSize
RAS170-C01Pre-coated Anti-SARS-CoV-2 Spike S1 (B.1.1.529) Antibody Microplate1 plate
RAS170-C02SARS-CoV-2 Spike S1 (B.1.1.529) Standard30 μg
RAS170-C03HRP-Anti-SARS-CoV-2 Spike S1 Antibody20 μg
RAS170-C0410xWashing Buffer 50 mL
RAS170-C052xDilution Buffer50 mL
RAS170-C06Substrate Solution12 mL
RAS170-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike S1 is urgently needed to accelerate the development of COVID-19 vaccines.
  • Application

    The kit is developed for specific detection of SARS-CoV-2 Spike S1 (B.1.1.529) in vaccine samples, which can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of Omicron-specific boosters.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format,providing a rapid detection of SARS-CoV-2 Spike S1.The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike S1 Antibody,SARS-CoV-2 Spike S1 as Control,HPR-Anti-SARS-CoV-2 Spike S1 Antibody and buffers.

    Your experiment will include 5 simple steps:

    a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

    b) Add the sample and Control diluted by Dilution Buffer to the plate.

    c) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to Ds document for the assay protocol.
 Spike S1 TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 218,400

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