ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > Protein > PNGase F > PNF-E51H3

PNGase F (500U/ul)

  • Product Details
    PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
  • Application
    1. Leaves N-glycan core oligosaccharides intact and suitable for further analysis
    2. Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
  • Unit Definition
    One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µl.
  • Quility Control
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 Mm Tris, 50 mM NaCl, 5 mM EDTA, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt.
    2. -70°C for 12 months under sterile conditions.
  • Notes

    To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.

    PNGase F will not cleave N-linked glycans containing core α1-3 Fucose.

SDS-PAGE
PNGase F SDS-PAGE

PNGase F (500U/ul) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Clinical and Translational Updates

コメント (0)


ETD of in-stock products: 4 business days

価格(JPY) : 30,800

価格(JPY) : 123,200

人気商品のおすすめ
抗His抗体

価格(JPY) : 67,200

価格(JPY) : 442,400

コンジュゲート HRP Anti-His 抗体

価格(JPY) : 70,000

価格(JPY) : 470,400

プロモーション&展示



データシート&ドキュメンテーション

DMF請求
DMF (Drug Master File)

関連製品一覧

新製品

お問い合わせ&コメント

This web search service is supported by Google Inc.

totopphone