GMP Biotinylated Human CD5 Protein, His,Avitag™

Premium grade (Pre-GMP) version CD5-H82E5 is now available for seamless transition.
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GMP-CD5H24-500ug
$4,715.00
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GMP-CD5H24-10mg (500ug X 20)
$52,360.00
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Product Features and Advantages
  • Wide-ranging functions: Acts as a high-quality target protein reagent to support biotin-streptavidin-based immunodetection, cell isolation, and functional screening workflows, enabling specific capture and analysis of CD5-expressing immune cells (e.g., T cells, B cells) in CAR-T, TCR-T, and TIL development, while serving as a validated tool for antibody discovery and epitope mapping. ACROBiosystems is the only GMP Biotinylated Human CD5 Protein, His, Avitag available supplier.
  • Comprehensive Safety Assurance: ECACC-compliant HEK293 host cell origin and comprehensive testing (28 items), animal origin-free (AOF) production process, with stringent final product quality control and release specifications to support clinical-grade applications.
GMP Platform Advantages
  • Quality Assurance: Global QMS with comprehensive and stringent QC release criteria.
  • End-to-End GMP Compliance: Full manufacturing and QC under a cGMP system.
  • Comprehensive Control of Adventitious Agents: Stringent biosafety from cell banks to final release.
  • Comprehensive Regulatory Support: Includes RSF and DMF to meet global requirements.
  • Resilient Supply Chain: Intelligent modular facilities ensure a stable global supply.
  • Professional Support: Extensive manufacturing and application expertise to accelerate development.
Source

GMP Biotinylated Human CD5 Protein, His,Avitag (GMP-CD5H24) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Pro 372 (Accession # P06127-1).

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 42.2 kDa. The protein migrates as 52 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

N-terminal Sequence Analysis

Arg-Leu-Ser-Trp-Tyr-Asp-Pro-Asp-Phe-Gln-Ala-Arg-Leu-Thr-Arg
(Routinely tested)

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotinylation

As determined by Quantitative ELISA binding assay against streptavidin.

Endotoxin

Less than 10 EU/mg, tested by the LAL method in compliance with USP <85> and Ph. Eur. 2.6.14.

Host Cell Protein

<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA

<0.02 ng/μg of protein tested by qPCR.

Sterility

Sterility testing was performed using the membrane filtration method in compliance with USP <71> and Ph. Eur. 2.6.1.

Mycoplasma

Negative

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.

Contact us for customized product form or formulation.

Vial Specification

6R (20 mm neck finish)

Shipping and Storage

This product is supplied and shipped on blue ice.

Upon receipt, store it immediately at -20°C or lower for long term storage.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 5 years in lyophilized state;
  • -70°C for 12 months under sterile conditions after reconstitution.

Background

CD5 protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes, and a subset of B lymphocytes. It serves to mitigate activating signals from the BCR, allowing B-1 cells to be activated only by strong stimuli. Additionally, CD5 is involved in regulating lipid synthesis and inflammatory responses.