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ClinMax™ Human IL-12/IL-23 p40 ELISA Kit

For research use only.
Product Details
Assay TypeSandwich-ELISA
AnalyteIL-12/IL-23 p40
Format96T
ReactivityHuman
Sensitivity<15.625 pg/mL
Standard Curve Range31.25 pg/mL-2000 pg/ml
Assay Time2hr
Suitable Sample TypeFor the quantitative determination of human IL-12/IL-23 p40 in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Materials Provided
IDComponentsSize
CEA203-C01Pre-coated Anti- IL-12/IL-23 p40 Antibody Microplate1 plate
CEA203-C02Human IL-12/IL-23 p40 Standard20μg ×2
CEA203-C03Biotin-Anti-IL-12/IL-23 p40 Antibody Con. Solution100 μL
CEA203-C04Biotin-Antibody Dilution Buffer8 mL
CEA203-C05Streptavidin-HRP Con. Solution500 uL
CEA203-C06Streptavidin-HRP Dilution Buffer15 mL
CEA203-C0720× Washing Buffer50 mL
CEA203-C08Sample Dilution Buffer15 mL ×2
CEA203-C09Substrate Solution12 mL
CEA203-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-12/IL-23 p40 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of IL-12/IL23 p40 in human serum and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     IL-12/IL-23 p40 Assay Principles
Typical Data Please refer to DS document for the assay protocol.
 IL-12/IL-23 p40 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-12/IL-23 p40 is less than 15.625 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different IL-12/IL-23 p40 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 IL-12/IL-23 p40 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of IL-12/IL-23 p40 were tested in independent assays. Acceptable criteria: CV<15%.

 IL-12/IL-23 p40 INTER-ASSAY STATISTICS
Recovery

Recombinant IL-12/IL-23 p40 was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-12/IL-23 p40 for serum samples is 103.2%.

 IL-12/IL-23 p40 RECOVERY
  • Background: IL-12/IL-23 p40
    Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL-12 and IL-23 are heterodimers that share the p40 subunit, and both cytokines are critical in the differentiation of T helper (Th)1 and Th17 cells, respectively.
  • Clinical and Translational Updates

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