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Human iPSC-Derived Cardiac Organoid Maintenance Kit

For research use only.
Materials Provided
Box A(2-8°C)
IDComponentsSize
RIPO-HWM004-C01Basal medium M-M225ml
 
Box B(-20°C)
IDComponentsSize
RIPO-HWM004-1-C01Supplement M-M25ml
  • Product Details
    Cardiac organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the human heart. Human iPSC-Derived Cardiac Organoid maintenance Kit allows the maturation and the long-term maintenance of cardiac organoids.
  • Product Specification
    The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect cardiac organoid differentiation.
  • Storage

    The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
    The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
    The opened kit should be stored according to components table.

Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of cardiac organoid differentiation.

Validation Data
Organoid Histology and State
 - Organoid Histology And State

The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) show regular beating from day 7-13 of differentiation.

Marker Expression
 - MARKER EXPRESSION

The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) demonstrate the expression of cardiomyocyte marker cTnT (green).

Organoid Activity
 - ORGANOID ACTIVITY

The electrical activity of the cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) was recorded using silicon probes (Ca. No. P64-7), revealing electrophysiological activity that closely resembles that of the actual human heart.

  • Clinical and Translational Updates

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