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ClinMax™ Human TSLP ELISA Kit

For research use only.
Product Details
Assay TypeSandwich-ELISA
AnalyteTSLP
Format96T
ReactivityHuman
Sensitivity<31.25pg/mL
Standard Curve Range31.25 pg/mL-2000 pg/mL
Assay Time1 hr 45 min
Suitable Sample TypeFor the quantitative determination of human TSLP in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Materials Provided
IDComponentsSize
CEA074-C01Pre-coated Anti-TSLP Antibody Microplate1 plate
CEA074-C02Human TSLP Standard20 ug×2
CEA074-C03Biotin-Anti-TSLP Antibody Con. Solution100 μL
CEA074-C04Biotin-Antibody Dilution Buffer8 mL
CEA074-C05Streptavidin-HRP Con. Solution500 μL
CEA074-C06Streptavidin-HRP Dilution Buffer15 mL
CEA074-C0720× Washing Buffer50 mL
CEA074-C08Sample Dilution Buffer15 mL×2
CEA074-C09Substrate Solution12 mL
CEA074-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human TSLP that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human TSLP in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     TSLP Assay Principles
Typical Data Please refer to DS document for the assay protocol.
 TSLP TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of TSLP is less than 31.25 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different TSLP concentrations were tested in one assay. Acceptable criteria: CV<10%.

 TSLP INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of TSLP were tested in independent assays. Acceptable criteria: CV<15%.

 TSLP INTER-ASSAY STATISTICS
Recovery

Recombinant TSLP was spiked into 3 human serum samples, and then analyzed. The average recovery of TSLP for serum samples is 91.5%.

 TSLP RECOVERY
  • Clinical and Translational Updates

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