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Your Position: ホーム > Protein > ALCAM > CD6-H5228

Human ALCAM Protein, His Tag

  • Synonym
    CD166,ALCAM,CD6L,KG-CAM,MEMD
  • Source
    Human ALCAM, His Tag(CD6-H5228) is expressed from human 293 cells (HEK293). It contains AA Trp 28 - Ala 526 (Accession # Q13740-1).
    Predicted N-terminus: Trp 28
  • Molecular Characterization
    ALCAM Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 57.8 kDa. The protein migrates as 70-100 kDa under reducing (R) condition, and 66-100 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ALCAM SDS-PAGE

Human ALCAM, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 ALCAM ELISA

Immobilized Human ALCAM, His Tag (Cat. No. CD6-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD6 Protein, His,Avitag (Cat. No. CD6-H82E3) with a linear range of 0.02-5 μg/mL (Routinely tested).

  • Background
    CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as Activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.
  • Clinical and Translational Updates

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