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Your Position: ホーム > Protein > CD200 > OX2-H82F1

Biotinylated Human CD200 / OX-2 Protein, Fc,Avitag™

  • シノニム
    CD200,MOX1,MOX2,MRC,OX-2,My033
  • ソース
    Biotinylated Human CD200, Fc,Avitag (OX2-H82F1) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gly 232 (Accession # P41217-1).
    Predicted N-terminus: Gln 31
  • 分子特性
    Online(Gln 31 - Gly 232) P41217-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 51.4 kDa. The protein migrates as 65-76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD200, Fc,Avitag (Cat. No. OX2-H82F1) SDS-PAGE gel

Biotinylated Human CD200, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD200, Fc,AvitagBiotinylated Human CD200, Fc,Avitag (Cat. No. OX2-H82F1) ELISA bioactivity

Immobilized Human CD200 R1, His Tag (Cat. No. CR2-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD200, Fc,Avitag (Cat. No. OX2-H82F1) with a linear range of 0.2-2 ng/mL (QC tested).

  • バックグラウンド
    CD200 is also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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