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Rhesus macaque PD-1 / PDCD1 Protein, His Tag (MALS verified)

  • シノニム
    PDCD1,PD1,CD279,SLEB2
  • ソース
    Rhesus macaque PD-1, His Tag (PD1-R52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ2-1).
    Predicted N-terminus: Leu 25
  • 分子特性
    Online(Leu 25 - Gln 167) B0LAJ2-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 17.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) SDS-PAGE gel

Rhesus macaque PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Rhesus macaque PD-1, His Tag (Cat. No. ) MALS images

The purity of Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) is more than 95% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Bioactivity-ELISA
Rhesus macaque PD-1, His TagRhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) ELISA bioactivity

Immobilized Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) at 2 μg/mL (100 μL/well) can bind nivolumab with a linear range of 0.1-3 ng/mL (QC tested).

Rhesus macaque PD-1, His TagRhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) ELISA bioactivity

Immobilized Biotinylated Human PD-L2, Fc,Avitag (Cat. No. PD2-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Rhesus macaque PD-1, His TagRhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) ELISA bioactivity

Immobilized Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) with a linear range of 0.078-0.625 μg/mL (Routinely tested).

  • バックグラウンド
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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