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Biotinylated Human BTLA (31-150) (M105V, G138S, V148M) Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    BTLA,CD272
  • Source
    Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag(BTA-H82F5) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1 (M105V,G138S,V148M)).
    Predicted N-terminus: Lys 31
  • Molecular Characterization
    BTLA Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 41.9 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
BTLA SDS-PAGE

Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
BTLA MALS images

The purity of Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag (Cat. No. BTA-H82F5) is more than 85% and the molecular weight of this protein is around 108-128 kDa verified by SEC-MALS.

Bioactivity-ELISA
 BTLA ELISA

Immobilized Human HVEM, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. HVM-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag (Cat. No. BTA-H82F5) with a linear range of 0.001-0.313 μg/mL (QC tested).

 BTLA ELISA

Immobilized Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag (Cat. No. BTA-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HVEM, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. HVM-H5255) with a linear range of 1-20 ng/mL (Routinely tested).

  • Background
    B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
  • Clinical and Translational Updates

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