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Alexa Fluor™ 488-Labeled Human CD7 Protein, His TagStar Staining

  • Synonym
    CD7,GP40,TP41,LEU-9,Tp40
  • Source
    Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (CD7-HA2H9) is produced via conjugation of AF488 to Human CD7 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD7 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).
    Predicted N-terminus: Ala 26
  • Molecular Characterization
    CD7 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 33.3 kDa.

  • Conjugate

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • Protein Ratio
    The AF488 to protein molar ratio is 0.9-1.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 CD7 ELISA

Immobilized Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) at 1 μg/mL (100 μL/well) can bind Anti-CD7 antibody, Mouse IgG1 with a linear range of 0.05-2 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.

Bioactivity-FACS
 CD7 FACS

1e5 of Mouse Anti-CD7 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).

 CD7 FACS

1e5 of Mouse Anti-CD7 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).

Bioactivity-Stability
 CD7 STABILITY

Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) is stable at 25℃ for 48 hours, equivalent to store at -70℃ for 2 years and freezing and thawing 3 times without performance reduction.

 CD7 STABILITY

Binding activity of three different lots of Alexa Fluor 488-Labeled Human CD7 Protein, His Tag against Anti-CD7 CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.

  • Background
    T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
  • Clinical and Translational Updates

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価格(USD) : 500.00

価格(USD) : 3235.00

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