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Alexa Fluor™ 488-Labeled Human CD7 Protein, His TagStar Staining

  • Synonym
    CD7,GP40,TP41,LEU-9,Tp40
  • Source
    Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (CD7-HA2H9) is produced via conjugation of AF488 to Human CD7 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD7 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).
    Predicted N-terminus: Ala 26
  • Molecular Characterization
    CD7 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 33.3 kDa.

  • Conjugate

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Evaluation of CAR expression
FACS Analysis of Anti-CD7 CAR Expression
 CD7 CAR_T

5e5 of anti-CD7 CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).

FACS Analysis of Non-specific binding to PBMCs
 CD7 CAR_T

5e5 of PBMCs were stained with Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa Fluor 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

  • Background
    T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 117,600

価格(JPY) : 732,480

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