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Alexa Fluor™ 555-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His TagStar Staining

  • Synonym
    GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C
  • Source
    Alexa Fluor 555-Labeled Human GUCY2C Protein, His Tag (GUC-HA2H6) is produced via conjugation of AF555 to Human GUCY2C Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human GUCY2C Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).
    Predicted N-terminus: Ser 24
  • Molecular Characterization
    GUCY2C Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 62.9 kDa.

  • Conjugate

    AF555

    Excitation Wavelength: 561 nm

    Emission Wavelength: 572 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Evaluation of CAR expression
FACS Analysis of Anti-GUCY2C CAR Expression
 GUCY2C CAR_T

5e5 of anti-GUCY2C CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 555-Labeled Human GUCY2C Protein, His Tag (Cat. No. GUC-HA2H6) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa 555 signal was used to evaluate the binding activity (QC tested).

FACS Analysis of Non-specific binding to PBMCs
 GUCY2C CAR_T

5e5 of PBMCs were stained with Alexa Fluor 555-Labeled Human GUCY2C Protein, His Tag (Cat. No. GUC-HA2H6) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa 555 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

  • Background
    GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
  • Clinical and Translational Updates

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価格(JPY) : 117,600

価格(JPY) : 732,480

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