ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > Kits > TNF-alpha > EP-165

TNF-alpha : TNFR1[HRP] Inhibitor Screening ELISA Kit-Pre coated plate

For research use only.
IDComponentsSize
EP165-C01Human TNF-alpha coated Plate1 plate
EP165-C02Anti-TNF-alpha Neutralizing Antibody10 μg
EP165-C03HRP-Human TNFR110 μg
EP165-C0420xWashing Buffer 50 mL
EP165-C051×Dilution Buffer50 mL
EP165-C06Substrate Solution12 mL
EP165-C07Stop Solution7 mL
  • Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • Application

    This kit is developed for screening for inhibitors of human TNF-alpha binding to human TNFR1.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new TNF-alpha pathway inhibitors. The assay takes advantage of our in house-developed binding of HRP human TNFR1 to immobilized human TNF-alpha in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human HRP TNFR1 protein, a human TNF-alpha protein, an anti-TNF-alpha neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human TNF-alpha.

    b) Add your molecule of interest to the tests.

    c) Add HRP-human TNFR1 to bind the coated TNF-alpha.

    d) Add TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to TNF-alpha: Osteoprotegerin binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to Ds document for the assay protocol.
 TNF-alpha TYPICAL DATA

INHIBITION OF TNF-alpha: TNFR1[HRP] BINDING BY ANTI- TNF-alpha NEUTRALIZING ANTIBODY
Serial dilutions of ANTI- TNF-alpha Neutralizing Antibody (Catalog # EP165-C02) (1:1 serial dilution, from 5μg/mL to 0.002441406 μg/mL) was added into TNF-alpha: TNFR1[HRP] binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

  • Clinical and Translational Updates

コメント (0)


ETD of in-stock products: 4 business days

価格(JPY) : 182,000

プロモーション&展示



医薬品開発状況

  • Number of Launched Drugs:61 Details
  • Number of Drugs in Clinical Trials:63 Details
  • Latest Research Phase:Approved

データシート&ドキュメンテーション

DMF請求
DMF (Drug Master File)

新製品

お問い合わせ&コメント

This web search service is supported by Google Inc.

totopphone