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Human iPSC-Derived Intestinal Organoid Maintenance Kit

For research use only.
Materials Provided
Box A(2-8°C)
IDComponentsSize
RIPO-IWM006-C01Intestinal organoid Basal medium MM225ml
 
Box B(-20°C)
IDComponentsSize
RIPO-IWM006-1-C01Intestinal organoid Supplement MM25ml
  • Product Details
    Intestinal organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human Intestinal regions. Human iPSC-Derived Intestinal Organoid Maintenance Kit allows long-term maturation and maintenance of the Intestinal organoids.
  • Product Specification
    The basic medium of this kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
  • Storage

    The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
    The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
    The opened kit should be stored according to components table.

Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of Intestinal organoid differentiation.

Validation Data
Organoid Histology and State
 - Organoid Histology And State

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.

Orgnaoid Histology
 - ORGNAOID HISTOLOGY

Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 Intestinal organoids.

Marker Expression
 - MARKER EXPRESSION

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2), brush borders (Villin) and endothelial cells (CD31).

 - MARKER EXPRESSION

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).

 - MARKER EXPRESSION

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).

 - MARKER EXPRESSION

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).

Organoid Activity
 - ORGANOID ACTIVITY

Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.

  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 51,800

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