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> CMC Production Process of Adoptive NK Cell Therapies
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CMC Production Process of Adoptive NK Cell Therapies
Activation and expansion of NK cells from different sources
Enhancement of NK cell activity
Activation and expansion of NK cells from different sources
NK cell activation products
Anti-4-1BB AntibodyComing Soon
iPSC/ESC Source
PBMC Source
UCB Source
NK Cell Lines Source
GMP Grade DLL4, VCAM1：Eliminate the need of feeder cells in culture systems and support differentiation of iPSCs to NK cells
Validated activity by iPSC/ESC to NK cell differentiation
Production and quality control are carried out under strict GMP systems and comply with regulations from multiple countries
Comprehensive quality release verification, with 16 quality control indicators
Enhanced safety (sterile, no mycoplasma, no exogenous virus, animal free production system, and various impurities residual detection)
Produced in a Pharmaceutical-grade production facility
Support for online and offline audits
Completion of FDA Drug Master File (DMF) registration
The combination of DLL4 (GMP-DL4H28) & VCAM1 (GMP-VC1H25) could significantly facilitate the differentiation efficiency of CD56+ CD3- NK cells.
SCF(GMP-SCFH25), Flt3L(GMP-FLLH28), IL-7(GMP-L07H24) could significantly promote the HSPC differentiation to NK cells, comparable to Company P.
Recommended cytokines combination：IL-2+IL-15 IL-15+IL-21 IL-2+IL-18
Validated activity by PBMC to NK cell differentiation
Production and quality control are carried out under strict GMP systems and comply with regulations from multiple countries
Comprehensive quality release verification, with 16 quality control indicators
Enhanced safety (sterile, no mycoplasma, no exogenous virus, animal free production system, and various impurities residual detection)
Produced in a Pharmaceutical-grade production facility
Support for online and offline audits
Completion of FDA Drug Master File (DMF) registration
Human PBMCs were cultured with IL-2 (Cat. No. GMP-L02H14), IL-15 (Cat. No. GMP-L15H13), IL-18 (Cat. No. IL8-H5114), IL-21 (Cat. No. GMP-L21H25). The result shows that they are functional and can promote the expansion of these cells with a reasonable cell viability.
Human PBMCs were cultured with IL-2 (Cat. No. GMP-L02H14), IL-15 (Cat. No. GMP-L15H13), IL-18 (Cat. No. IL8-H5114), IL-21 (Cat. No. GMP-L21H25). The result shows that they are functional and can increase the percentage of the CD3-CD56+ cells.
Validated activity by UCB to NK cell differentiation
Production and quality control are carried out under strict GMP systems and comply with regulations from multiple countries
Comprehensive quality release verification, with 16 quality control indicators
Enhanced safety (sterile, no mycoplasma, no exogenous virus, animal free production system, and various impurities residual detection)
Pharmaceutical-grade production facility
Support for online and offline audits
Completion of FDA Drug Master File (DMF) registration
Production and quality control are carried out under strict GMP systems and comply with regulations from multiple countries
Comprehensive quality release verification, with 16 quality control indicators
Enhanced safety (sterile, no mycoplasma, no exogenous virus, animal free production system, and various impurities residual detection)
Pharmaceutical-grade production facility
Support for online and offline audits
Completion of FDA Drug Master File (DMF) registration
Enhancement of NK cell activity
High purity, high enzyme activity, high cleavage efficiency
Possesses nuclear localization signals to enhance editing efficiency
Aseptic, ultra-low endotoxin
Produced in GMP-compliant facilities and undergoes QC testing
Different amounts of Cas9 were incubated with the same amount of excess gRNA and plasmid for 60 minutes at 37°C. When using 400-200 ng Acro Cas9, the cutting efficiency is greater than 90%. In comparison, when using a 200 ng Competitor T, the cutting efficiency is only about 50%.
Resources
Cytokines residue ELISA kits
IL-2, IL-4, IL-6, IL-7, IL-10, IL-15, IL-21, IL-1B, TNF-alpha, GM-CSF residue kits.
HCD detection
Enzyme residue kit
DNase Activity Assay Kit (Fluorescence)
Mono-growth factor detection
Multiplex detection
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