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Ready-to-use Human iPSC-Derived Liver Organoids

  • Product Details
    The Ready-to-use liver organoids are derived form human iPS cells and being cultured for more than 60 days. Organoids generated using Human iPSC-Derived liver organoid feature various types of cells, including hepatocytes, bile duct cells and vascular endothelial cells.
  • Product Specification
    The live organoids are ready-to-use organoids that are delivered in shipping medium and has to go through a 48h recovery process according to instruction.
  • Storage

    After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.

*Please note that to support full process of organoid culture and maintenance, this product have to be used with Human iPSC-Derived Liver Organoid Expansion and Maintenance Medium (Cat. No.RIPO-RWM010),you can follow this link for product information.
Product Diagram
 Organoids PRODUCT DIAGRAM

Protocol Diagram of liver organoid differentiation.

Validation Data
Orgnaoid Histology
 Organoids ORGNAOID HISTOLOGY

H&E Staining of liver organoid.

Marker Expression
 Organoids MARKER EXPRESSION

The liver organoids show expression of hepatocytes (HNF4a), hepatobiliary tracts (CK19), Albumin (ALB) and vascular endothelial cell (CD31).

 Organoids MARKER EXPRESSION

Pre-mature liver organoids show liver lobules-like structure.

Organoid Activity
 Organoids ORGANOID ACTIVITY

Liver organoids show better performance than HepG2 cell line in terms of the expression of Albumin, CYP3A4, CYP1A2, CYP2C9, CYP7A1 and BSEP.

 Organoids ORGANOID ACTIVITY

Liver organoid show response to Rifampicin treatment as indication by the increase fluorescence intensity of CYP3A4.

  • Clinical and Translational Updates

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