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Product Details
Source
PE-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
Antibody Type
Recombinant Monoclonal
Clone
P3C09
Isotype
Rabbit IgG, Kappa
Host Species
Rabbit
Reactivity
Human
Immunogen
Purified 218 linker protein
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Specificity
This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.
Application
Flow Cytometry (Evaluation of CAR and SCFV containing 218 linker on Human cells).
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-FACS
Flow cytometric analysis of Anti-BCMA(C11D5.3) CAR 293 cells staining with PE-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (Cat. No.218-PCFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). PE signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody binding to CD3+ cells present in human PBMC. 5e5 of humanPBMCs were simultaneously stained with FITC anti-CD3 antibody and PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, RabbitIgG (P3C09) (Cat. No. 218-PCFMY3) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity tohuman CD3+ cells (QC tested).
Protocol
Compared Data
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-PCFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and Company C. PE signal was used to evaluate the binding activity. The biological activity level of 218-PCFMY3 is superior to Company C (Routinely tested).
Protocol
Typical Data
Immunohistochemical analysis of paraffin embedded anti-BCMA CAR-T cells labelled with 218-PCFMY3.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with lHC staining protocol.
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