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Product Details
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PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-HPY53) is a neutralizing antibody produced via site-specific conjugation of PE to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a proprietary technology. We also carry another proclin-containing version of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-PY54A2) produced with the same production process except for proclin, and the proclin-containing product has the same performance and can be stored under 2-8 °C for no less than 12 months after reconstitution.
Besides, we carry another premium grade PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (FM3-PY54G0), produced with the same production process but under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. It is designed for cell isolation and cell culture applications in the early preclinical stage.
Clone
Y45
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Recombinant FMC63 scFv derived from HEK293 cells
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
Application
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). Please note that this product is NOT compatible to streptavidin detection system.
Recommended Dilution
1:50
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.5% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS
5e5 of the Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) and PE-Labeled Monoclonal Mouse IgG1 Antibody Isotype Control (Cat. No. DNP-PM1) respectively, PE signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
FACS Analysis of Blood Samples
After Fc receptor blockade, whole blood only control (Figure A) and whole blood spiked with CAR-T cells (Figure B) were first stained with PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation) (Preservative free) (Cat. FM3-HPY53), following 7-AAD staining. The data were analyzed using FCS Express 7 software (Routinely tested). CAR-T cells were identified as PE-positive events within the live CD45+CD3+ (or CD45+) lymphocyte population. The proportion of CD45+CAR+ cells is around 20%, which is largely consistent with the theoretical spike-in value.
Protocol
Customer Reviews Writing Reviews
H***e2026-03-28
LikesMy team has been using the Anti-FMC63 Antibody, and they always have consistent and reliable data that is being used for preclinical IND-enabling studies as well as clinical studies. I recommend using AcroBiosystems for your antibody needs.- X***g2026-03-28 LikesOur bioanalytical team have consistently obtained reliable CAR detection results using the PE‑labeled monoclonal anti‑FMC63 antibody in our animal studies. The antibody shows strong specificity, clear signal, and low background, with consistent performance across runs. Overall, it has been a dependable, high‑quality reagent, and we consider the vendor a reliable supplier for CAR detection assays.
- M***y2022-09-27 LikesWe started using this antibody to assess CAR expression in Jurkat cells and it has proven to work perfectly for what we need. We've used it in flow cytomerty anaylsis to great success. It means we can co-stain easily for other markers to ensure our CAR expression is consistent and not lost. Our group has decided this antibody will be our primary method of confirming CAR expression going forward and allows us to determine transduction efficiency easily.
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