Product Details
Source
Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
1A2
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
virus
Immunogen
Recombinant HSV-1 (strain KOS) post-gB Protein is expressed from human 293 cells
Specificity
Specifically recognizes HSV-1 (strain KOS) post-gB Protein.
Application
ApplicationRecommended UsageWestern Blot10 μg/mLPurification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Immobilized HSV-1 (strain KOS) post-gB Protein, His Tag (Cat. No. POB-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (Cat. No. HSB-MY2113) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. GLB-H52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (Cat. No. HSB-MY2113) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Western Blot

Detection of Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) by Western Blot. Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) at 10 μg/mL dilution +HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. POB-V52H3) & HSV-1 (strain KOS) post-gB Protein, His Tag (Cat. No. GLB-H52H3) at 400 ng.<br>
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
Predicted band size: 95 kDa 12% Bis-Tris Protein Gel.
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