Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (MALS verified)

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Cat. No. / Size
Price
Qty
HSB-MY2113-100ug
$400.00
HSB-MY2113-1mg (500ug X 2)
$2655.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    1A2

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    virus

  • Immunogen

    Recombinant HSV-1 (strain KOS) post-gB Protein is expressed from human 293 cells

  • Specificity

    Specifically recognizes HSV-1 (strain KOS) post-gB Protein.

  • Application

    Application
    Recommended Usage
    Western Blot
    10 μg/mL
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     gB ELISA

    Immobilized HSV-1 (strain KOS) post-gB Protein, His Tag (Cat. No. POB-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (Cat. No. HSB-MY2113) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  •  gB ELISA

    Immobilized HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. GLB-H52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (Cat. No. HSB-MY2113) with a linear range of 0.06-2 ng/mL (Routinely tested).

    Protocol
  • Western Blot

     gB WESTERN BLOT
  • Detection of Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) by Western Blot. Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) at 10 μg/mL dilution +HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. POB-V52H3) & HSV-1 (strain KOS) post-gB Protein, His Tag (Cat. No. GLB-H52H3) at 400 ng.<br>
    Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
    Predicted band size: 95 kDa 12% Bis-Tris Protein Gel.

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Background

Herpes simplex viruses (HSV)-1 and HSV-2 cause a variety of human diseases, including encephalitis, keratitis, neonatal disease, and mucocutaneous and skin diseases such as herpes labialis, genital herpes, and herpetic whitlow. HSV-1 gB, which is a class III fusion glycoprotein, is a major target of antibody-mediated immunity (21). It plays an essential role in the entry of the virus into a host cell, together with other HSV-1 envelope glycoproteins, including gD and a complex of gH and gL (gH/gL).

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