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Product Details
Source
Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
1A4
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Influenza A
Immunogen
Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] HA is expressed from human 293 cells
Specificity
Specifically recognizes Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein.
Application
ApplicationRecommended UsageWestern Blot2-0.1 ug/mLCross Verification
This product No cross-reactivity in ELISA with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.4-13 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Western Blot
Detection of monoclonal Anti-Influenza A HA(H5N1),Influenza A (A/Hong Kong/483/1997) antibody-1A4, Human IgG1 | Human Kappa ,HEK by Western Blot. monoclonal Anti-Influenza A HA(H5N1), Influenza A (A/Hong Kong/483/1997) antibody-1A4, Human IgG1 | Human Kappa, HEK at 0.1ug/ml dilution + Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (MALS verified), His Tag at 400ng.<br>
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
Predicted band size: 80 kDa 12% Bis-Tris Protein Gel.
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