Product Details
Source
PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.
Antibody Type
Hybridoma Monoclonal
Clone
55F8
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
His tag (N-terminal, C-terminal and internal His-tagged fusion proteins)
Immunogen
Purified his-tagged Protein
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Specificity
Specifically binds to His-tagged proteins without recognizing non-tagged proteins.
Application
Flow Cytometry (Detection the His-tagged protein).
Recommended Dilution
1:50
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 3 months after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS

Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with 100 μL of 10 μg/mL Human CD19 (20-291) Protein, His Tag (Cat. No. CD9-H52H2) and negative control protein respectively, washed and then followed with PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) (Cat. No. HIS-PFY63) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
Protocol
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