Biotinylated Mouse IgG1 Isotype Control (Biotin, mAb, carrier free, MALS verified)

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製品番号/仕様
価格
数量
DNP-BM1-25ug
$35.00
DNP-BM1-200ug
$115.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Source

    Biotinylated Mouse IgG1 Isotype Control (mAb) is a mouse monoclonal antibody recombinantly expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Conjugate

    Biotin

  • Specificity

    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Bioactivity-ELISA

     IgG control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) with a linear range of 1-39 ng/mL (QC tested).

    Protocol
  • Bioactivity-FACS

     IgG control FACS

    2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

    Protocol
  •  IgG control FACS

    2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

    Protocol
  •  IgG control FACS

    5e5 of PBMCs were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

    Protocol

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バックグランド

Fluorescently or biotin labeled isotype controls are used to assess non specific staining and autofluorescence in flow cytometry, ELISA, and imaging assays. By recognizing DNP, a hapten absent in biological samples, these controls provide a reliable baseline to distinguish background fluorescence or signal from true antigen specific binding.

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